Any feedback?
Literature summary extracted from
- Soluble methane monooxygenase from Methylococcus capsulatus Bath (1990), Methods Enzymol., 188, 181-190.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.25 | protein A unstable to successive freezing and thawing | Methylococcus capsulatus |
| 1.14.13.25 | protein B, in crude form requires addition of protease inhibitor phenylmethylsulfonyl fluoride | Methylococcus capsulatus |
| 1.14.13.25 | protein C requires presence of thiol protective agent, e.g. sodium thioglycolate throughout purification | Methylococcus capsulatus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | Acetylene | - |
Methylococcus capsulatus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.25 | additional information | - |
additional information | - |
Methylococcus capsulatus | |
| 1.14.13.25 | 0.003 | - |
methane | - |
Methylococcus capsulatus | |
| 1.14.13.25 | 0.0168 | - |
O2 | - |
Methylococcus capsulatus | |
| 1.14.13.25 | 0.0558 | - |
NADH | with methane | Methylococcus capsulatus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.25 | cytoplasm | enzyme from cells grown under conditions of low copper availability | Methylococcus capsulatus | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | Iron | protein A, hydroxylase component: contains a binuclear iron center | Methylococcus capsulatus | |
| 1.14.13.25 | Iron | protein C, reductase component: contains 1 [Fe2-S2] | Methylococcus capsulatus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | additional information | - |
see under subunits: molecular weights of the subunits of components | Methylococcus capsulatus |
| 1.14.13.25 | additional information | - |
enzyme system consists of 3 protein components A, B, C | Methylococcus capsulatus |
| 1.14.13.25 | 17000 | - |
component: protein B, gel filtration | Methylococcus capsulatus |
| 1.14.13.25 | 38000 | 38550 | component: protein C, gel filtration | Methylococcus capsulatus |
| 1.14.13.25 | 220000 | - |
component: protein A, gel filtration | Methylococcus capsulatus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | methane + NAD(P)H + O2 | Methylococcus capsulatus | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | Methylococcus capsulatus Bath | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | methanol + NAD(P)+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.25 | Methylococcus capsulatus | - |
Bath | - |
| 1.14.13.25 | Methylococcus capsulatus Bath | - |
Bath | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.25 | - |
Methylococcus capsulatus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | mechanism | Methylococcus capsulatus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | additional information | - |
- |
Methylococcus capsulatus |
| 1.14.13.25 | 0.265 | - |
protein A after reconstitution of iron | Methylococcus capsulatus |
| 1.14.13.25 | 6 | - |
purified protein C | Methylococcus capsulatus |
| 1.14.13.25 | 11.37 | - |
purified protein B | Methylococcus capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | ammonia + NAD(P)H + O2 | - |
Methylococcus capsulatus | hydroxylamine + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | benzene + NAD(P)H + H+ + O2 | - |
Methylococcus capsulatus | phenol + hydroquinone + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | chloromethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | formaldehyde + NAD(P)+ + H2O + ? | - |
? | |
| 1.14.13.25 | CO + NAD(P)H + O2 | - |
Methylococcus capsulatus | CO2 + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | CO + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | CO2 + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | cyclohexane + NAD(P)H + O2 | - |
Methylococcus capsulatus | cyclohexanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | dichloromethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | CO + Cl- + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | dimethyl ether + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + formaldehyde + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | dimethyl ether + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + formaldehyde + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | ethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | ethanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | ethene + NAD(P)H + O2 | - |
Methylococcus capsulatus | epoxyethane + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | hexane + NAD(P)H + O2 | - |
Methylococcus capsulatus | 1-hexanol + 2-hexanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | naphthalene + NAD(P)H + O2 | - |
Methylococcus capsulatus | alpha-naphthol + beta-naphthol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | styrene + NAD(P)H + O2 | - |
Methylococcus capsulatus | styrene epoxide + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | styrene + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | styrene epoxide + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | trans-2-butene + NAD(P)H + O2 | - |
Methylococcus capsulatus | trans-2,3-epoxybutane + trans-2-buten-1-ol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | trichloromethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | CO2 + Cl- + NAD(P)+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | ? | protein A: 2 * 54000-60630, alpha+ 2 * 42000-44720, beta + 2 * 17000-19840, gamma | Methylococcus capsulatus |
| 1.14.13.25 | More | see under molecular weight for the size of the protein components | Methylococcus capsulatus |
| 1.14.13.25 | More | enzyme system consists of 3 protein components A, B, C | Methylococcus capsulatus |
| 1.14.13.25 | More | component A is a hydroxylase | Methylococcus capsulatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | MMO | - |
Methylococcus capsulatus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 45 | - |
assay at | Methylococcus capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | FAD | protein C, reductase component: contains 1 mol FAD per mol protein | Methylococcus capsulatus | |
| 1.14.13.25 | NADH | preferred | Methylococcus capsulatus | |
| 1.14.13.25 | NADPH | - |
Methylococcus capsulatus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
