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Literature summary extracted from

  • Tassaneeyakul, W.; Veronese, M.E.; Birkett, D.J.; Gonzalez, F.J.; Miners, J.O.
    Validation of 4-nitrophenol as an in vitro substrate probe for human liver CYP2E1 using cDNA expression and microsomal kinetic techniques (1993), Biochem. Pharmacol., 46, 1975-1981.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.29 alpha-naphthoflavone 60% activiation at 25 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.29
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.29 4-nitrophenol mutual competitive inhibition with chlorzoxazone Homo sapiens
1.14.13.29 alpha-naphthoflavone slight Homo sapiens
1.14.13.29 chlorzoxazone mutual competitive inhibition with 4-nitrophenol Homo sapiens
1.14.13.29 coumarin slight Homo sapiens
1.14.13.29 diethyldithiocarbamate 50% inhibition at 0.002-0.003 mM Homo sapiens
1.14.13.29 mephenytoin slight Homo sapiens
1.14.13.29 quinidine slight Homo sapiens
1.14.13.29 sulfaphenazole slight Homo sapiens
1.14.13.29 troleandomycin slight Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.29 0.021
-
 4-nitrocatechol cDNA expressed enzyme Homo sapiens
1.14.13.29 0.03
-
 4-nitrocatechol
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.29 microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.29 40000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.29 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.29
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.29 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.29 4-nitrophenol + NADH + O2
-
 Homo sapiens 4-nitrocatechol + NAD+ + H2O
-
 ?
1.14.13.29 chlorzoxazone + NADH + O2 6-hydroxylation Homo sapiens ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.29 0.042
-
 4-nitrophenol inhibition of chlorzoxazone 6-hydroxylation Homo sapiens
1.14.13.29 0.047
-
 chlorzoxazone inhibition of 4-nitrophenol hydroxylation Homo sapiens