Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Prieto, M.A.; Perez-Aranda, A.; Garcia, J.L.
    Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range (1993), J. Bacteriol., 175, 2162-2167.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.9 expression in Escherichia coli K-12 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.9 additional information W21, 4-hydroxyphenylacetate deficient mutant Escherichia coli

General Stability

EC Number General Stability Organism
1.14.14.9 rather unstable in solution Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.9 59000
-
2 * 59000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 Escherichia coli 4-hydroxyphenylacetic acid degradative pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.9 Escherichia coli
-
strain W, ATCC 11105 derived from ATCC 9637
-
1.14.14.9 no activity in Escherichia coli K-12
-
DH1 CECT 416, ATCC 27325
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.9 difficult because of the low stability of the enzyme in solution Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.9 2,5-dihydroxyphenylacetate + ? 155% of 4-hydroxyphenylacetate activity Escherichia coli ?
-
?
1.14.14.9 3,4-dihydroxyphenylacetate + ? 65% of 4-hydroxyphenylacetate Escherichia coli ?
-
?
1.14.14.9 3-hydroxyphenylacetate + NADH + O2 82% activity of 4-hydroxyphenylacetate Escherichia coli 3,4-hydroxyphenylacetate + NAD+ + H2O
-
?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2
-
Escherichia coli 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 4-hydroxyphenylacetic acid degradative pathway Escherichia coli 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
1.14.14.9 additional information rather broad substrate specificity, acting on mono- and dihydric phenols. Several substrates are below 50% of activity Escherichia coli ?
-
?
1.14.14.9 p-cresol + ? 51% of 4-hydroxyphenylacetate activity Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.9 dimer 2 * 59000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.9 FAD no stimulation Escherichia coli
1.14.14.9 NADH dependent, not NADPH dependent Escherichia coli