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Literature summary extracted from
- Purification and properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Corynebacterium cyclohexanicum (1985), Eur. J. Biochem., 147, 97-104.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.33 | 2,4-Dihydroxybenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum |  |
| 1.14.13.33 | 2,5-Dihydroxybenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum | |
| 1.14.13.33 | 2-Hydroxybenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum | |
| 1.14.13.33 | 3-hydroxybenzoate | - |
Corynebacterium cyclohexanicum | |
| 1.14.13.33 | 4-Aminobenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum | |
| 1.14.13.33 | 4-Fluorobenzoate | - |
Corynebacterium cyclohexanicum | |
| 1.14.13.33 | Cl- | non-competitive | Corynebacterium cyclohexanicum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.33 | 0.035 | - |
4-hydroxybenzoate | - |
Corynebacterium cyclohexanicum | |
| 1.14.13.33 | 0.045 | - |
NADH | pH 7.0-8.4 | Corynebacterium cyclohexanicum | |
| 1.14.13.33 | 0.063 | 0.17 | NADPH | Km increases as pH rises from 7.0 to 8.4 | Corynebacterium cyclohexanicum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.33 | soluble | - |
Corynebacterium cyclohexanicum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.33 | Mg2+ | required for full activity in low concentrations of phosphate buffer, most effective at 5 mM | Corynebacterium cyclohexanicum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.33 | 47000 | - |
PAGE of native and denatured enzyme | Corynebacterium cyclohexanicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.33 | Corynebacterium cyclohexanicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.33 | - |
Corynebacterium cyclohexanicum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.33 | 30.5 | - |
NADPH | Corynebacterium cyclohexanicum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.33 | -20°C, several months | Corynebacterium cyclohexanicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.33 | 4-hydroxybenzoate + NAD(P)H + O2 | enzyme is highly specific for 4-hydroxybenzoate, but uses NADH and NADPH at approximately equal rates | Corynebacterium cyclohexanicum | 3,4-dihydroxybenzoate + NAD(P)+ + H2O | - |
? | |
| 1.14.13.33 | 4-hydroxybenzoate + NADH + H+ + O2 | - |
Corynebacterium cyclohexanicum | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.33 | monomer | 1 * 47000, PAGE of native and denatured enzyme | Corynebacterium cyclohexanicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.33 | 50 | - |
- |
Corynebacterium cyclohexanicum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.33 | 60 | - |
10 min, stable | Corynebacterium cyclohexanicum |
| 1.14.13.33 | 65 | - |
rapid inactivation above, p-hydroxybenzoate protects against heat inactivation | Corynebacterium cyclohexanicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.33 | 7.1 | - |
NADPH | Corynebacterium cyclohexanicum |
| 1.14.13.33 | 7.8 | - |
NADH | Corynebacterium cyclohexanicum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.33 | 6.2 | 8.8 | pH 6.2: about 50% of activity maximum, pH 8.8: about 70% of activity maximum, NADPH | Corynebacterium cyclohexanicum |
| 1.14.13.33 | 6.5 | 8.8 | 50% of activity maximum at pH 6.5 and pH 8.8, NADH | Corynebacterium cyclohexanicum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.33 | 4.3 | 8.5 | 4°C, 24 h, stable | Corynebacterium cyclohexanicum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.33 | FAD | flavoprotein, 0.8 FAD per enzyme molecule | Corynebacterium cyclohexanicum | |
| 1.14.13.33 | NADH | - |
Corynebacterium cyclohexanicum | |
| 1.14.13.33 | NADPH | - |
Corynebacterium cyclohexanicum | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.33 | 0.029 | 0.045 | Cl- | - |
Corynebacterium cyclohexanicum | |
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