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Literature summary extracted from

  • Rao, P.V.S.; Sreeleela, N.S.; Kumar, R.P.; Vaidyanathan, C.S.
    Anthranilic acid hydroxylase (Aspergillus niger) (1970), Methods Enzymol., 17A, 510-513.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.13.35 glutathione stabilizes Aspergillus niger
1.14.13.35 the enzyme is inactivated by treatment with ammonium sulfate or by adsorption on DEAE-cellulose or CM-cellulose, filtration through Sephadex G-25 or dialysis against 0.025 M sodium phosphate buffer, pH 7, containing 1 mM GSH irreversibly inactivates the enzyme Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.35 1,10-phenanthroline
-
 Aspergillus niger
1.14.13.35 2,2'-dipyridyl
-
 Aspergillus niger
1.14.13.35 diethyldithiocarbamate
-
 Aspergillus niger
1.14.13.35 heavy metal ions
-
 Aspergillus niger
1.14.13.35 N-ethylmaleimide
-
 Aspergillus niger
1.14.13.35 NaN3
-
 Aspergillus niger
1.14.13.35 p-chloromercuribenzoate
-
 Aspergillus niger
1.14.13.35 Salicylaldoxime
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.35 0.15
-
 anthranilic acid
-
 Aspergillus niger
1.14.13.35 0.16
-
 NADPH
-
 Aspergillus niger

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.13.35 Acetone inactivates the enzyme Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.35 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.35 using protamine sulfate treatment, DEAE-cellulose treatment and alumina C-gamma treatment Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.35 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.35 additional information
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.14.13.35 -20°C, 50% inactivation of the purified enzyme after 2 days Aspergillus niger
1.14.13.35 frozen, mycelium, no appreciable loss of activity after 1 week Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.35 3-hydroxyanthranilate + NADPH + O2 no activity Aspergillus niger ?
-
 ?
1.14.13.35 3-methylanthranilate + NADPH + O2 no activity Aspergillus niger ? + NADP+ + NH3
-
 ?
1.14.13.35 anthranilate + NADPH + O2
-
 Aspergillus niger 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?
1.14.13.35 additional information no activity with: 3-hydroxyanthranilic acid, benzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, m-aminobenzoic acid, p-aminobenzoic acid, methylanthranilate or ethylanthranilate Aspergillus niger ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.35 8.2
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.35 7.5 9.5 at pH 7.5 or pH 9.0 about 60% of activity maximum Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.35 additional information 2% of the activity when NADPH is replaced by NADH, no effect: FAD, FMN Aspergillus niger
1.14.13.35 NADPH absolute requirement for NADPH Aspergillus niger