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Literature summary extracted from

  • Taylor, D.G.; Trudgill, P.W.
    Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453 (1986), J. Bacteriol., 165, 489-497.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.108 2,2'-bipyridine dissociates FMN from oxygenating enzyme component Pseudomonas putida
1.14.14.108 p-hydroxymercuribenzoate oxygenase coupled to NADH oxidase Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.108 cytoplasm
-
 Pseudomonas putida 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.108 Fe2+ no Fe2+ in dehydrogenase Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.108 additional information
-
 2 electrophoretic components, PAGE at pH 8.9 and higher Pseudomonas putida
1.14.14.108 37000
-
 2 * 37000, oxygenating component E1, SDS-PAGE Pseudomonas putida
1.14.14.108 76000
-
 oxygenating component E1, equilibrium ultracentrifugation Pseudomonas putida
1.14.14.108 120000
-
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.108 Pseudomonas putida
-
-
-
1.14.14.108 Pseudomonas putida C1 / ATCC 17453
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.108
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.108 3.56
-
 oxygenating component Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O i.e. cyclopentenoic acid ?
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida C1 / ATCC 17453 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O i.e. cyclopentenoic acid ?
1.14.14.108 (+)-bornanone + FMNH2 + O2 (+)-camphor Pseudomonas putida 1,2-campholide + FMN + H2O
-
 ?
1.14.14.108 (+)-bornanone + FMNH2 + O2 (+)-camphor Pseudomonas putida C1 / ATCC 17453 1,2-campholide + FMN + H2O
-
 ?
1.14.14.108 additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?
1.14.14.108 additional information rubredoxin not mentioned Pseudomonas putida C1 / ATCC 17453 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.108 dimer 2 * 37000, oxygenating component E1, SDS-PAGE Pseudomonas putida
1.14.14.108 More 2 electrophoretic components, PAGE at pH 8.9 and higher Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.108 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.108 7.2
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.108 FMN dialyzable, not covalently bound Pseudomonas putida