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Literature summary extracted from

  • Woo, S.L.C.; Gillam, S.S.; Woolf, L.I.
    The isolation and properties of phenylalanine hydroxylase from human liver (1974), Biochem. J., 139, 741-749.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.14.16.1 phenylalanine stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.1 4-chloromercuribenzoate 1 mM, complete inhibition after 10 min Homo sapiens
1.14.16.1 4-Chlorophenylalanine competitive vs. phenylalanine Homo sapiens
1.14.16.1 4-Fluorophenylalanine above 1 mM Homo sapiens
1.14.16.1 copper-chelating agents
-
 Homo sapiens
1.14.16.1 diethyldithiocarbamate 1 mM, 58% inhibition Homo sapiens
1.14.16.1 DL-alpha-tocopherol strong inhibition Homo sapiens
1.14.16.1 Iron-chelating agents
-
 Homo sapiens
1.14.16.1 Thiol-binding reagents
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.318
-
 L-phenylalanine
-
 Homo sapiens
1.14.16.1 1
-
 4-Fluorophenylalanine approx. value Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.1 54000
-
 2 * 54000, fetal liver enzyme, SDS-PAGE Homo sapiens
1.14.16.1 110000
-
 fetal liver enzyme, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1 fetal, newborn and adult enzyme are probably identical Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.1 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.1 0.225
-
 liver enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 4-fluorophenylalanine + tetrahydrobiopterin + O2
-
 Homo sapiens ?
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Homo sapiens L-tyrosine + dihydrobiopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.1 dimer 2 * 54000, fetal liver enzyme, SDS-PAGE Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 tetrahydrobiopterin
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.16.1 1.1
-
 4-Chlorophenylalanine
-
 Homo sapiens