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Literature summary extracted from

  • Fujisawa, H.; Okuno, S.
    Tyrosine 3-monooxygenase from rat adrenals (1987), Methods Enzymol., 142, 63-71.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.16.2 EDTA, 0.1 mM stabilizes Rattus norvegicus
1.14.16.2 glycerol, 25% stabilizes Rattus norvegicus
1.14.16.2 Tween 80, 0.05% stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 Catecholamines
-
 Rattus norvegicus
1.14.16.2 dopamine
-
 Rattus norvegicus
1.14.16.2 epinephrine
-
 Rattus norvegicus
1.14.16.2 norepinephrine
-
 Rattus norvegicus
1.14.16.2 O2
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 0.058
-
 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.2 59000
-
 4 * 59000, SDS-PAGE Rattus norvegicus
1.14.16.2 260000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 Rattus norvegicus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine ?
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Rattus norvegicus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 adrenal medulla
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 1604
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.16.2 -80°C, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 3 months Rattus norvegicus
1.14.16.2 4°C, 24 h, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 68% loss of activity Rattus norvegicus
1.14.16.2 4°C, 5 h, without stabilizing agents, almost complete loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Rattus norvegicus ?
-
 ?
1.14.16.2 L-tyrosine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropterin + O2 synthetic pterin as electron donor Rattus norvegicus ?
-
 ?
1.14.16.2 L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2 synthetic pterin as electron donor Rattus norvegicus ?
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.2 tetramer 4 * 59000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.2 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.2 additional information
-
 pH profile is affected by a variety of conditions: enzymatic phosphorylation by cAMP-dependent protein kinase, calmodulin-dependent protein kinase II and presence of polyanions Rattus norvegicus
1.14.16.2 5.4 5.5 crude extract Rattus norvegicus
1.14.16.2 6 7.5 purified enzyme Rattus norvegicus