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Literature summary extracted from
- Characterization of yellowfin tuna (Thunnus albacares, Scombroidei) tryptophan hydroxylase (1997), Comp. Biochem. Physiol. B, 116, 161-165.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.4 | CaCl2 | 1 mM, 7% activation | Thunnus albacares |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.4 | CoCl2 | 1 mM, complete inhibition | Thunnus albacares | |
| 1.14.16.4 | D,L-4-chlorophenylalanine | - |
Thunnus albacares | |
| 1.14.16.4 | ZnSO4 | 1 mM, 80% inhibition | Thunnus albacares | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.4 | 0.023 | - |
L-tryptophan | - |
Thunnus albacares | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 96000 | - |
4 * 96000, SDS-PAGE | Thunnus albacares |
| 1.14.16.4 | 280000 | - |
liver enzyme, gel filtration | Thunnus albacares |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.4 | Thunnus albacares | - |
sp. scombroidei, yellofin tuna | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.4 | acid treatment, ammonium sulfate, Sepharose CL-6B, DEAE-sepharose, butyl-Sepharose, Toyopearl | Thunnus albacares |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.16.4 | liver | - |
Thunnus albacares | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 13.3 | - |
liver enzyme | Thunnus albacares |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | - |
Thunnus albacares | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.16.4 | tetramer | 4 * 96000, SDS-PAGE | Thunnus albacares |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 40 | - |
- |
Thunnus albacares |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 35 | - |
stable below | Thunnus albacares |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 8 | - |
- |
Thunnus albacares |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 5 | 10 | enzyme retains more than 80% activity after 60 min at 4°C between pH 7.0 and pH 7.6, unstable above pH 10.0 and below pH 5.0 | Thunnus albacares |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.4 | 0.021 | - |
D,L-4-chlorophenylalanine | - |
Thunnus albacares | |
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