Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nakata, H.; Fujisawa, H.
    Tryptophan 5-monooxygenase from mouse mastocytoma P815. A simple purification and general properties (1982), Eur. J. Biochem., 124, 595-601.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.4 dithiothreitol or other sulfhydryl reagents like 2-mercaptoethanol and L-cysteine are essential for activation, 5fold increase in activity Mus musculus

General Stability

EC Number General Stability Organism
1.14.16.4 EDTA stabilizes Mus musculus
1.14.16.4 ethylene glycol, 50%, stabilizes Mus musculus
1.14.16.4 NaCl: 1 M, in addition to other stabilizing agents brings a great improvement in stabilization Mus musculus
1.14.16.4 Tween 20 stabilizes Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.4 1,10-phenanthroline
-
 Mus musculus
1.14.16.4 2,2'-dipyridyl
-
 Mus musculus
1.14.16.4 3,4-dihydroxyphenylalanine
-
 Mus musculus
1.14.16.4 3,4-Dihydroxyphenylethylamine
-
 Mus musculus
1.14.16.4 4-Fluorotryptophan
-
 Mus musculus
1.14.16.4 5-Fluorotryptophan
-
 Mus musculus
1.14.16.4 5-hydroxytryptophan
-
 Mus musculus
1.14.16.4 6-Fluorotryptophan
-
 Mus musculus
1.14.16.4 8-hydroxyquinoline
-
 Mus musculus
1.14.16.4 L-4-chlorophenylalanine
-
 Mus musculus
1.14.16.4 L-p-Chlorophenylalanine
-
 Mus musculus
1.14.16.4 L-phenylalanine competitive vs. tryptophan Mus musculus
1.14.16.4 L-tryptophan competitive to L-phenylalanine Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 0.045
-
 L-tryptophan cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Mus musculus
1.14.16.4 0.055
-
 2-amino-4-hydroxy-6-methyltetrahydropteridine substrate L-tryptophan Mus musculus
1.14.16.4 0.1
-
 L-phenylalanine cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.4 Fe2+ 5fold increase in activity Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.4 53000
-
 4 * 53000, enzyme from mastocytoma cells, SDS-PAGE Mus musculus
1.14.16.4 270000
-
 enzyme from mastocytoma cells, gel filtration Mus musculus
1.14.16.4 280000
-
 enzyme from mastocytoma cells, gradient PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.4 mastocytoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 5.28
-
 purified from mastocytoma cells Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-phenylalanine + tetrahydropteridine + O2
-
 Mus musculus tyrosine + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2
-
 Mus musculus ?
-
 r
1.14.16.4 L-tryptophan + tetrahydropteridine + O2
-
 Mus musculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.4 tetramer 4 * 53000, enzyme from mastocytoma cells, SDS-PAGE Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.16.4 4
-
 complete loss of activity in absence of stabilizing agents after 20 h Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.4 7.2
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.16.4 6 8.7 about 50% activity at pH 6.0 and 8.7 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.16.4 0.026
-
 L-4-chlorophenylalanine
-
 Mus musculus