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Literature summary extracted from
- Bovine adrenal medullary dopamine beta-hydroxylase: purification by affinity chromatography, kinetic studies and presence of essential histidyl residues (1973), Biochim. Biophys. Acta, 327, 313-327.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.1 | diethyldicarbonate | - |
Bos taurus |  |
| 1.14.17.1 | diethyldithiocarbamate | - |
Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.17.1 | 0.55 | - |
tyramine | - |
Bos taurus | |
| 1.14.17.1 | 1.25 | - |
ascorbate | - |
Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.17.1 | chromaffin granule | - |
Bos taurus | 42583 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.1 | Cu2+ | a copper protein | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.1 | 280000 | - |
SDS-PAGE with 0.01% SDS | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.17.1 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.17.1 | - |
Bos taurus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.17.1 | dopamine + 2 ascorbate + O2 = noradrenaline + 2 monodehydroascorbate + H2O | ping pong mechanism | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.17.1 | adrenal medulla | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.1 | 3.9 | - |
- |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.17.1 | -30°C, 10 mM potassium phosphate buffer, pH 6.5, stable for at least 1 month | Bos taurus |
| 1.14.17.1 | 0-4°C, 10-15% loss of activity in 24 h | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.1 | 3,4-dihydroxyphenethylamine + ascorbate + O2 | - |
Bos taurus | noradrenaline + dehydroascorbate + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.1 | 50 | - |
- |
Bos taurus |
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Release 2023.1 (January 2023)
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