Literature summary extracted from

Long, R.A.; Weppelman, R.M.; Taylor, J.E.; Tolman, R.L.; Olson, G.
Purification and characterization of avian dopamine beta-hydroxylase (1981), Biochemistry, 20, 7423-7431.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.14.17.1	acetate	-	Gallus gallus
1.14.17.1	dehydroascorbate	-	Gallus gallus
1.14.17.1	fumarate	-	Gallus gallus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.14.17.1	ascorbate	-	Gallus gallus
1.14.17.1	norepinephrine	-	Gallus gallus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.17.1	0.2	-	dopamine	-	Gallus gallus
1.14.17.1	2	-	ascorbate	-	Gallus gallus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.17.1	chromaffin granule	-	Gallus gallus	42583	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.14.17.1	NaCl	-	Gallus gallus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.17.1	80000	-	4 * 80000, SDS-PAGE after treatment with 2-mercaptoethanol, subunits joined in pairs by disulfide bonds	Gallus gallus
1.14.17.1	316000	-	gel filtration	Gallus gallus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.17.1	Gallus gallus	-	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
1.14.17.1	glycoprotein	-	Gallus gallus

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.17.1	-	Gallus gallus

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
1.14.17.1	dopamine + 2 ascorbate + O2 = noradrenaline + 2 monodehydroascorbate + H2O	uni-uni bi-uni ping pong mechanism	Gallus gallus	a

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.14.17.1	adrenal gland	-	Gallus gallus	-

Storage Stability

EC Number	Storage Stability	Organism
1.14.17.1	-20°C, stable for at least 6 weeks	Gallus gallus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.17.1	3,4-dihydroxyphenethylamine + ascorbate + O2	3,4-dihydroxyphenethylamine is identical with 4-(2-aminoethyl)-1,2-benzendiol i.e. dopamine, ferricyanide can replace ascorbate	Gallus gallus	noradrenaline + dehydroascorbate + H2O	norepinephrine	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.17.1	tetramer	4 * 80000, SDS-PAGE after treatment with 2-mercaptoethanol, subunits joined in pairs by disulfide bonds	Gallus gallus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.14.17.1	-	4	24 h, 10-15% loss of activity	Gallus gallus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.14.17.1	5	6	-	Gallus gallus

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.14.17.1	4.5	6.5	sharp decrease in activity between pH 4.5 and 5.0 and between pH 6.0 and 6.5	Gallus gallus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.14.17.1	5	-	norepinephrine	-	Gallus gallus
1.14.17.1	20	-	ascorbate	-	Gallus gallus

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Release 2023.1 (January 2023)