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Literature summary extracted from

  • Chikuma, T.; Kocha, T.; Hanaoka, K.; Kato, T.; Ishii, Y.; Tanaka, A.
    Characterization of peptidylglycine alpha-amidating monooxygenase in bovine hypothalamus (1994), Neurochem. Int., 25, 349-354.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.3 diethyldithiocarbamate copper chelator Bos taurus
1.14.17.3 additional information activity is not affected by pepstatin A, phenylmethylsulfonyl fluoride, and soybean trypsin inhibitor Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.3 0.022
-
 dabsyl-Gly-Phe-Gly form 2 Bos taurus
1.14.17.3 0.143
-
 dabsyl-Gly-Phe-Gly form 1 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ optimal activity at 0.016 mM Bos taurus
1.14.17.3 Cu2+ Cu2+ is absolutely required for optimal activity Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.3 49000
-
 form 1, gel filtration Bos taurus
1.14.17.3 69000
-
 form 2, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Bos taurus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 hypothalamus 2 forms, distinguishable by molecular weight Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + ascorbate + O2
-
 Bos taurus 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.3 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.3 6
-
 2 optima: at pH 6.0 and at pH 7.5 Bos taurus
1.14.17.3 7.5
-
 2 optima: at pH 6.0 and at pH 7.5 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Bos taurus