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Literature summary extracted from

  • Glembotski, C.C.
    Further characterization of the peptidyl alpha-amidating enzyme in rat anterior pituitary secretory granules (1985), Arch. Biochem. Biophys., 241, 673-683.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.17.3 repeated freeze-thaw cycles have no effect on enzyme activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.3 ascorbate above 0.4 mM for the purified enzyme, above 1.5 mM for the enzyme in crude extract Rattus norvegicus
1.14.17.3 Cu2+ at higher concentration Rattus norvegicus
1.14.17.3 diethyldithiocarbamate copper chelator Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.3 additional information
-
 additional information Km for D-Tyr-Val-Gly depends on concentration of ascorbate, Km for ascorbate is constant over a wide range of D-Tyr-Val-Gly concentration Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.17.3 secretory granule
-
 Rattus norvegicus 30141
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ a copper protein Rattus norvegicus
1.14.17.3 Cu2+ Cu2+ is absolutely required for optimal activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.3 50000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 peptidylglycine + ascorbate + O2 Rattus norvegicus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.3 partially Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 pituitary gland anterior, intermediate and posterior Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 adrenocorticotrophic hormone(9-14) + ascorbate + O2
-
 Rattus norvegicus adrenocorticotrophic hormone(9-13)-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 alpha-N-acetyl-adrenocorticotrophic hormone(1-14) + ascorbate + O2
-
 Rattus norvegicus alpha-N-acetyl-adrenocorticotrophic hormone(1-13)-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
 ?
1.14.17.3 additional information substrates are also physiologically relevant peptides related to alpha-melanotropine Rattus norvegicus ?
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2
-
 Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
1.14.17.3 peptidylglycine + ascorbate + O2 COOH-terminal glycine Rattus norvegicus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.3 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.17.3 4
-
 incubation for 12-15 h, 47% loss of activity Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.3 ascorbate dependent on Rattus norvegicus