Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cahoon, E.B.; Coughlan, S.J.; Shanklin, J.
    Characterization of a structurally and functionally diverged acyl-acyl carrier protein desaturase from milkweed seed (1997), Plant Mol. Biol., 33, 1105-1110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.2 expression in Escherichia coli Asclepias syriaca
1.14.19.3 expression of N-terminal truncated enzyme in Escherichia coli Thunbergia alata

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.2 Asclepias syriaca
-
 milkweed
-
1.14.19.3 Thunbergia alata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.3 recombinant enzyme Thunbergia alata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.2 seed
-
 Asclepias syriaca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.2 palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 8% of activity with stearoyl-[acyl-carrier-protein], 7fold higher activity than the enzyme from castor Asclepias syriaca 9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?
1.14.19.2 stearoyl-[acyl-carrier protein] + reduced acceptor + O2
-
 Asclepias syriaca oleoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?
1.14.19.2 tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2 3% of activity with stearoyl-[acyl-carrier-protein], 30fold higher activity than castor enzyme Asclepias syriaca 9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.2 Ferredoxin
-
 Asclepias syriaca
1.14.19.3 Ferredoxin
-
 Thunbergia alata