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Literature summary extracted from
- Fat metabolism in higher plants. Properties of a soluble stearyl-acyl carrier protein desaturase from maturing Carthamus tinctorius (1974), Arch. Biochem. Biophys., 162, 158-165.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.2 | dithiothreitol | enhances activity | Carthamus tinctorius |  |
| 1.14.19.2 | reduced glutathione | enhances activity | Carthamus tinctorius | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.2 | beta-mercaptoethanol | - |
Carthamus tinctorius | |
| 1.14.19.2 | cytochrome c3 | - |
Carthamus tinctorius | |
| 1.14.19.2 | cytochrome c553 | - |
Carthamus tinctorius | |
| 1.14.19.2 | FAD | - |
Carthamus tinctorius | |
| 1.14.19.2 | FMN | - |
Carthamus tinctorius | |
| 1.14.19.2 | KCN | 1 mM, strong inhibition | Carthamus tinctorius | |
| 1.14.19.2 | additional information | not inhibited by CO | Carthamus tinctorius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.2 | 0.002 | - |
NADPH | - |
Carthamus tinctorius | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.2 | cytoplasm | - |
Carthamus tinctorius | 5737 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | Carthamus tinctorius | P22243 | safflower | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | specific for stearoyl-CoA | Carthamus tinctorius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | cotyledon | - |
Carthamus tinctorius | - |
| 1.14.19.2 | seed | - |
Carthamus tinctorius | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.2 | stearoyl-CoA + electron donor + O2 | - |
Carthamus tinctorius | 9-octadecenoyl-CoA + acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | no activity with NADH | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | electron donor NADPH | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | a system composed of ferredoxin, grana lamellae, ascorbic acid, dichlorophenolindophenol and light is the most effective reductant | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | enzyme is specific for stearoyl-CoA | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.2 | Ferredoxin | required for activity | Carthamus tinctorius | |
| 1.14.19.2 | additional information | NADH is ineffective as electron donor | Carthamus tinctorius | |
| 1.14.19.2 | NADPH | - |
Carthamus tinctorius | |
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Release 2023.1 (January 2023)
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