EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.3 | NADH-cytochrome b5 reductase | EC 1.6.2.2, absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
1.14.19.3 | NADPH-cytochrome b5 reductase | EC 1.6.2.2, absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
1.14.19.3 | O2 | absolutely essential for DELTA6-desaturation | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.3 | bathophenanthroline sulfonate | mild inhibition | Rattus norvegicus | |
1.14.19.3 | beta-mercaptoethanol | mild inhibition | Rattus norvegicus | |
1.14.19.3 | dithiothreitol | mild inhibition | Rattus norvegicus | |
1.14.19.3 | iron chelators | - |
Rattus norvegicus | |
1.14.19.3 | KCN | - |
Rattus norvegicus | |
1.14.19.3 | N-ethylmaleimide | only little effect | Rattus norvegicus | |
1.14.19.3 | p-chloromercuribenzene sulfonate | - |
Rattus norvegicus | |
1.14.19.3 | Tiron | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.3 | 0.045 | - |
linoleoyl-CoA | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.19.3 | microsome | membrane | Rattus norvegicus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.3 | Fe2+ | DELTA6-desaturase contains 15.1 nmol iron/mg protein | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.19.3 | 65000 | 68000 | gel filtration | Rattus norvegicus |
1.14.19.3 | 66000 | - |
SDS-PAGE | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.3 | linoleic acid + AH2 + O2 | Rattus norvegicus | - |
gamma-linolenic acid + A + H2O | - |
? | |
1.14.19.3 | linoleic acid + AH2 + O2 | Rattus norvegicus Wistar | - |
gamma-linolenic acid + A + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.3 | Rattus norvegicus | - |
- |
- |
1.14.19.3 | Rattus norvegicus Wistar | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.14.19.3 | phospholipoprotein | 20-40 M phospholipid per mol of protein | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.19.3 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.19.3 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.19.3 | 0.0344 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.19.3 | -70°C, stored under nitrogen the enzyme is unstable, loses 80% of its activity after repeated freezing and thawing | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.3 | linoleic acid + AH2 + O2 | - |
Rattus norvegicus | gamma-linolenic acid + A + H2O | - |
? | |
1.14.19.3 | linoleic acid + AH2 + O2 | - |
Rattus norvegicus Wistar | gamma-linolenic acid + A + H2O | - |
? | |
1.14.19.3 | linoleoyl-CoA + AH2 + O2 | - |
Rattus norvegicus | gamma-linolenoyl-CoA + A + 2 H2O | - |
? | |
1.14.19.3 | linoleoyl-CoA + AH2 + O2 | - |
Rattus norvegicus Wistar | gamma-linolenoyl-CoA + A + 2 H2O | - |
? | |
1.14.19.3 | octadeca-9,12-dienoic acid + AH2 + O2 | - |
Rattus norvegicus | gamma-linolenic acid + A + H2O | - |
? | |
1.14.19.3 | octadeca-9,12-dienoic acid + AH2 + O2 | - |
Rattus norvegicus Wistar | gamma-linolenic acid + A + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.19.3 | monomer | 1 * 66000, SDS-PAGE | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.19.3 | 7 | - |
- |
Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.19.3 | 6 | 9 | - |
Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.3 | cytochrome b5 | absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
1.14.19.3 | linoleoyl-CoA | absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
1.14.19.3 | NADH | absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
1.14.19.3 | NADPH | absolutely essential for DELTA6-desaturation | Rattus norvegicus |