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Literature summary extracted from

  • Bonnerjea, J.; Pontin, S.; Hoare, M.; Dunnill, P.
    Poly(ethylene glycol) precipitation and two aquaeous-phase separation of progesterone 11-alpha hydroxylase from Rhizopus nigricans (1988), Appl. Microbiol. Biotechnol., 27, 362-365.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.99.14 synthesis membrane-associated enzymes catalyse several reactions of industrial interest, including steroid hydroxylation Rhizopus stolonifer
1.14.99.14 synthesis immobilisation of membrane-bound multi-enzyme complexes for industrial use Rhizopus stolonifer

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.14 endoplasmic reticulum
-
 Rhizopus stolonifer 5783
-
1.14.99.14 membrane
-
 Rhizopus stolonifer 16020
-
1.14.99.14 microsome
-
 Rhizopus stolonifer
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.14 progesterone + NADPH + O2 Rhizopus stolonifer necessary step in the synthesis of cortico-steroid hormones 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.14 Rhizopus stolonifer
-
 ATCC 6277b
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.14 enzyme complex Rhizopus stolonifer

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.14 additional information multi-enzyme complex contains cytochrome P450 and NADPH cytochrome c reductase Rhizopus stolonifer ?
-
 ?
1.14.99.14 progesterone + NADPH + O2
-
 Rhizopus stolonifer 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 necessary step in the synthesis of cortico-steroid hormones Rhizopus stolonifer 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?