Any feedback?
Literature summary extracted from
- Interactions of substrates with a purified 4-methoxybenzoate monooxygenase system (O-demethylating) from Pseudomonas putida (1973), Eur. J. Biochem., 35, 126-134.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | Putidamonooxin | - |
Pseudomonas putida |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.99.15 | NADH, not NADPH or substrate stabilize the reductase | Pseudomonas putida |
| 1.14.99.15 | substrate or substrate analogues stabilize putidamomooxin by preventing loss of Fe2+ | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | 3-Methoxybenzoate | - |
Pseudomonas putida |  |
| 1.14.99.15 | 4-trifluoromethylbenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | benzoate | competitive inhibition | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.99.15 | 0.0014 | - |
4-Methoxybenzoate | - |
Pseudomonas putida | |
| 1.14.99.15 | 0.0019 | - |
O2 | + 4-methoxybenzoate | Pseudomonas putida | |
| 1.14.99.15 | 0.0038 | - |
O2 | + 3,4-dimethoxybenzoate | Pseudomonas putida | |
| 1.14.99.15 | 0.01 | - |
O2 | + 4-ethoxybenzoate | Pseudomonas putida | |
| 1.14.99.15 | 0.051 | - |
O2 | + N-methyl-4-aminobenzoate | Pseudomonas putida | |
| 1.14.99.15 | 0.055 | - |
O2 | + 4-methylbenzoate | Pseudomonas putida | |
| 1.14.99.15 | 0.077 | - |
N-methyl-4-aminobenzoate | - |
Pseudomonas putida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.15 | 4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.15 | Pseudomonas putida | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.15 | 0.002 | - |
4-hyroxybenzoate, reductase | Pseudomonas putida |
| 1.14.99.15 | 0.004 | - |
4-hydroxybenzoate, monooxygenase | Pseudomonas putida |
| 1.14.99.15 | 0.008 | - |
3-methoxybenzoate, 4-methoxybenzoate, cell-free extract | Pseudomonas putida |
| 1.14.99.15 | 0.009 | - |
N,N-dimethyl-4-aminobenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.021 | - |
4-hydroxy-3-methoxybenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.023 | - |
3-hydroxybenzoate, reconstituted enzyme | Pseudomonas putida |
| 1.14.99.15 | 0.024 | - |
4-ethoxybenzoate, N-methyl-4-aminobenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.025 | - |
3,4-dimethoxybenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.04 | - |
4-methoxybenzoate, cell-free extract | Pseudomonas putida |
| 1.14.99.15 | 0.055 | - |
4-hyxdroxybenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.077 | - |
4-hydroxybenzoate, reconstituted enzyme | Pseudomonas putida |
| 1.14.99.15 | 0.11 | - |
3-methoxybenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.178 | - |
N-methyl-4-aminobenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.314 | - |
4-ethoxybenzoate, piperonylate | Pseudomonas putida |
| 1.14.99.15 | 0.345 | - |
3,4-dimethoxybenzoate | Pseudomonas putida |
| 1.14.99.15 | 0.36 | - |
4-methoxybenzoate | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.15 | 3,4-dimethoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxy-3-methoxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 1.14.99.15 | 3,4-methylenedioxybenzoate + NADH + O2 | piperonylate | Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | protocatechuate | ? | |
| 1.14.99.15 | 3-hydroxybenzoate + NADH + O2 | partial uncoupler | Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | in the uncoupled part of the reaction, 3-hydroxybenzoate is not hydroxylated and H2O2 is a product, too | ? | |
| 1.14.99.15 | 3-methoxybenzoate + NADH + O2 | partial uncoupler | Pseudomonas putida | 3-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 1.14.99.15 | 4-ethoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + acetaldehyde + NAD+ + H2O | - |
? | |
| 1.14.99.15 | 4-Hydroxy-3-methoxybenzoate + NADH + O2 | vanillate, partial uncoupler | Pseudomonas putida | ? | - |
? | |
| 1.14.99.15 | 4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 1.14.99.15 | alkylbenzoates | - |
Pseudomonas putida | ? | - |
? | |
| 1.14.99.15 | additional information | - |
Pseudomonas putida | additional information | uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction | ? | |
| 1.14.99.15 | N,N'-dimethyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 1.14.99.15 | N-methyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.15 | 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.15 | 8 | - |
- |
Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.15 | FMN | - |
Pseudomonas putida | |
| 1.14.99.15 | NADH | - |
Pseudomonas putida | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
