EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.15 | Putidamonooxin | - |
Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.15 | Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.15 | 4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.15 | Pseudomonas putida | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.99.15 | 4-methoxybenzoate + reduced acceptor + O2 = 4-hydroxybenzoate + formaldehyde + acceptor + H2O | the bacterial enzyme is a two-component enzyme, consisting of an iron-sulfur flavoprotein (FMN), NADH-putidamonooxin-reductase and a ferredoxin-type, oxygen-activating protein, putidamonooxin | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.15 | 3-hydroxybenzoate + NADH + O2 | partial uncoupler | Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | in the uncoupled part of the reaction, 3-hydroxybenzoate is not hydroxylated and H2O2 is a product, too | ? | |
1.14.99.15 | 3-nitro-4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.99.15 | 4-hydroxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.99.15 | 4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.99.15 | 4-vinylbenzoate + NADH + O2 | external dioxygenase reaction by substrate induced modulation | Pseudomonas putida | 4-glycylbenzoate + NAD+ + H2O | - |
? | |
1.14.99.15 | additional information | overview: substrates that are not oxygenized while NADH-oxidation and O2-consumption are catalyzed, such as: benzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-hydroxybenzoate, 4-bromobenzoate, 2-aminobenzoate, 3-aminobenzoate, 4-trifluoromethylbenzoate, 4-tert-butylbenzoate | Pseudomonas putida | additional information | uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction | ? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.99.15 | 30 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.15 | NADH | - |
Pseudomonas putida |