Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Michihara, A.; Sawamura, M.; Nara, Y.; Ikeda, K.; Yamori, Y.
    Purification and characterization of two mevalonate pyrophosphate decarboxylases from rat liver: a novel molecular species of 37 kDa (1997), J. Biochem., 122, 647-654.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.33 0.02
-
 5-diphosphomevalonate 37000 MW enzyme form Rattus norvegicus
4.1.1.33 0.0227
-
 5-diphosphomevalonate 45000 MW enzyme form Rattus norvegicus
4.1.1.33 0.8
-
 ATP 37000 MW enzyme form Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.33 Co2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Rattus norvegicus
4.1.1.33 Co2+ Mn2+ and Co2+ are the best activators for the 45000 MW enzyme form Rattus norvegicus
4.1.1.33 Mg2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Rattus norvegicus
4.1.1.33 Mn2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Rattus norvegicus
4.1.1.33 Mn2+ Mn2+ is the best activator for the 37000 MW enzyme form Rattus norvegicus
4.1.1.33 Mn2+ Mn2+ and Co2+ are the best activators for the 45000 MW enzyme form Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.33 37000
-
 2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE Rattus norvegicus
4.1.1.33 45000
-
 2 * 45000, SDS-PAGE Rattus norvegicus
4.1.1.33 90000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.33 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.33 additional information intracellular glycosylation does not contribute to the difference between the 45000 MW form and the 37000 MW form of the enzyme Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.33 2 enzyme forms: MW 37000 and MW 45000. The MW 37000 enzyme form is induced by the diet containing cholestyramine and pravastin Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.33 liver from animals fed a diet containing cholestyramine and pravastin Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.33 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.33 ATP + 5-diphosphomevalonate
-
 Rattus norvegicus ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.33 dimer 2 * 45000, SDS-PAGE Rattus norvegicus
4.1.1.33 dimer 2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.33 7
-
 37000 MW form and 45000 MW form Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.33 4 9 about 35% of maximal activity at pH 4 and at pH 9, 37000 MW enzyme form Rattus norvegicus
4.1.1.33 6 9 pH 6: about 35% of maximal activity, pH 9: about 30% of maximal activity, 45000 MW enzyme form Rattus norvegicus