Any feedback?
Literature summary extracted from
- Solubilization and partial purification of heme oxygenase from rat liver (1977), J. Biol. Chem., 252, 5900-5903.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.18 | cysteine | 0.1 mM, slight stimulation, inhibition at 1 mM | Rattus norvegicus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.14.18 | 50% loss of activity during one cycle of freezing and thawing | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.18 | 2-mercaptoethanol | 0.1 mM, 81% inhibition | Rattus norvegicus | |
| 1.14.14.18 | 4-hydroxymercuribenzoate | 1 mM, complete inhibition | Rattus norvegicus | |
| 1.14.14.18 | cysteine | 1 mM, 67% inhibition | Rattus norvegicus | |
| 1.14.14.18 | dithiothreitol | 0.01 mM, 88% inhibition | Rattus norvegicus | |
| 1.14.14.18 | Hg2+ | 0.3 mM, complete inhibition | Rattus norvegicus | |
| 1.14.14.18 | iodoacetamide | 5 mM, 39% inhibition, 10 mM, 79% inhibition | Rattus norvegicus | |
| 1.14.14.18 | p-chloromercuribenzoate | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.18 | 0.04 | - |
heme | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.18 | microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | 68000 | - |
x * 68000, possibly a trimer, SDS-PAGE | Rattus norvegicus |
| 1.14.14.18 | 180000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.18 | heme + 2 NADPH + 2 H+ + 2 O2 | Rattus norvegicus | involved in heme metabolism | biliverdin + Fe2+ + CO + 2 NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.18 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.18 | DEAE-cellulose, Sephadex G-200 | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.18 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | 12.7 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.18 | -20°C, 2 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.18 | heme + 2 NADPH + 2 H+ + 2 O2 | involved in heme metabolism | Rattus norvegicus | biliverdin + Fe2+ + CO + 2 NADP+ + H2O | - |
? | |
| 1.14.14.18 | heme + electron donor + O2 | electron donor NADH | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
| 1.14.14.18 | heme + electron donor + O2 | NADPH is more effective than NADH | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
| 1.14.14.18 | heme + electron donor + O2 | electron donor NADPH | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
| 1.14.14.18 | methemoglobin + electron donor + O2 | 30% of activity with heme | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.18 | ? | x * 68000, possibly a trimer, SDS-PAGE | Rattus norvegicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | additional information | - |
no activity at 0°C | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | 60 | - |
5 min, complete loss of activity | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.18 | 7.5 | - |
- |
Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.18 | NADH | NADPH is more effective than NADH | Rattus norvegicus | |
| 1.14.14.18 | NADPH | NADPH is more effective than NADH | Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
