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Literature summary extracted from

  • Lin, M.W.; Lin, M.T.; Lin, C.T.
    Copper/zinc-superoxide dismutase from lemon cDNA and enzyme stability (2002), J. Agric. Food Chem., 50, 7264-7270.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 Cu,Zn-SOD, expression in Escherichia coli as His-tagged protein, DNA and amino acid sequence analysis Citrus limon

General Stability

EC Number General Stability Organism
1.15.1.1 dimeric form is more stable against proteolysis than monomeric form Citrus limon

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+
-
 Citrus limon
1.15.1.1 Zn2+
-
 Citrus limon

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Citrus limon Q71S31 Cu,Zn-SOD
-
1.15.1.1 Citrus limon CuZn-SOD Q71S31 Cu,Zn-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 2 forms, 1 dimer and 1 monomer Citrus limon
1.15.1.1 recombinant His-tagged Cu,Zn-SOD from Escherichia coli Citrus limon

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid sequence alignment and comparison Citrus limon
a
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 A metalloprotein. Enzymes from most eukaryotes contain both copper and zinc, those from mitochondria and most prokaryotes contain manganese or iron. ligand binding site and structure Citrus limon
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 fruit
-
 Citrus limon
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 additional information
-
 dimeric form shows 65% higher activity than monomeric form Citrus limon

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
 Citrus limon O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Citrus limon CuZn-SOD O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer
-
 Citrus limon
1.15.1.1 monomer
-
 Citrus limon
1.15.1.1 More Cu,Zn-SOD exists as 70% dimeric form and 30% monomeric form Citrus limon

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 90
-
 dimer: half-life 99 min, dimeric form is more stable than monomeric form Citrus limon

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 2.2 11 Cu,Zn-SOD, stable Citrus limon