Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, W.; Zhu, R.H.; Li, G.P.; Wang, D.C.
    cDNA cloning, high-level expression, purification, and characterization of an avian Cu,Zn superoxide dismutase from Peking duck (2002), Protein Expr. Purif., 25, 379-388.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 Cu,Zn-SOD, overexpression in Escherichia coli Anas platyrhynchos domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+
-
Anas platyrhynchos domestica
1.15.1.1 Zn2+
-
Anas platyrhynchos domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 16000
-
2 * 16000, Cu,Zn-SOD, SDS-PAGE Anas platyrhynchos domestica
1.15.1.1 32000
-
gel filtration Anas platyrhynchos domestica
1.15.1.1 32000
-
Cu,Zn-SOD Anas platyrhynchos domestica

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Anas platyrhynchos domestica
-
Cu,Zn-SOD
-
1.15.1.1 Anas platyrhynchos domestica
-
Peking duck
-
1.15.1.1 Anas platyrhynchos domestica CuZn-SOD
-
Cu,Zn-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 Cu,Zn-SOD, recombinant from Escherichia coli and native enzyme Anas platyrhynchos domestica

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid sequence alignment and comparison Anas platyrhynchos domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
Anas platyrhynchos domestica O2 + H2O2
-
?
1.15.1.1 O2- + H+
-
Anas platyrhynchos domestica CuZn-SOD O2 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 16000, Cu,Zn-SOD, SDS-PAGE Anas platyrhynchos domestica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 70
-
recombinant Cu,Zn-SOD, after 2 h 20% activity remaining, after 3 h all activity is lost Anas platyrhynchos domestica