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Literature summary extracted from

  • Lamarre, C.; LeMay, J.D.; Deslauriers, N.; Bourbonnais, Y.
    Candida albicans expresses an unusual cytoplasmic manganese-containing superoxide dismutase (SOD3 gene product) upon the entry and during the stationary phase (2001), J. Biol. Chem., 276, 43784-43791.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 Mn-SOD, expression in Escherichia coli as His-tagged protein, DNA sequence analysis Candida albicans
1.15.1.1 overexpression in Saccharomyces cerevisiae mutant lacking Cu,Zn-SOD, restores activity of the mutant Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 CN- slight inhibition, Mn-SOD Candida albicans
1.15.1.1 H2O2 no inhibition: Mn-SOD Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.15.1.1 cytoplasm SOD-I: Mn-SOD Candida albicans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Iron 0.4 mol per mol of mn-SOD Candida albicans
1.15.1.1 Mn2+ 0.9 Mn per mol of enzyme Candida albicans
1.15.1.1 additional information Mn-SOD contains as well Fe3+, but is only active with manganese Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 25400
-
 4 * 25400, Mn-SOD, SDS-PAGE Candida albicans
1.15.1.1 85000
-
 recombinant His-tagged Mn-SOD, gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 O2- + H+ Candida albicans Mn-SOD, expression is strongly stimulated during stationary phase in cell culture, enzyme is atypical and plays an important role in cell protection against reactive oxygen in the cytosol in the stationary phase O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Candida albicans Q96UT6 SOD-3 gene, Mn-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 Mn-SOD recombinant from Escherichia coli Candida albicans

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid sequence alignment and comparison Candida albicans
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.15.1.1 reconstitution of active protein after withdrawal of metal, higher activity with Mn2+, lower activity with Fe3+ Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 additional information
-
-
 Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
 Candida albicans O2 + H2O2
-
 ?
1.15.1.1 O2- + H+ Mn-SOD, expression is strongly stimulated during stationary phase in cell culture, enzyme is atypical and plays an important role in cell protection against reactive oxygen in the cytosol in the stationary phase Candida albicans O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 tetramer 4 * 25400, Mn-SOD, SDS-PAGE Candida albicans