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Literature summary extracted from

  • Lumsden, J.; Cammack, R.; Hall, D.O.
    Purification and physicochemical properties of superoxide dismutase from two photosynthetic microorganisms (1976), Biochim. Biophys. Acta, 438, 380-392.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.15.1.1 dimethyl sulfoxide: Spirulina enzyme is stable up to 55%, Rhodopseudomonas enzyme up to 70% v/v Cereibacter sphaeroides
1.15.1.1 dimethyl sulfoxide: Spirulina enzyme is stable up to 55%, Rhodopseudomonas enzyme up to 70% v/v Arthrospira platensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2 Fe-SOD Arthrospira platensis
1.15.1.1 H2O2 no inhibition: Mn-SOD Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Iron 1.0-1.45 mol per mol of enzyme Arthrospira platensis
1.15.1.1 Mn2+ 1 atom per subunit Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 18100
-
 2 * 18100, Fe-SOD, SDS-PAGE Arthrospira platensis
1.15.1.1 18300
-
 2 * 18300, Mn-SOD, SDS-PAGE Cereibacter sphaeroides
1.15.1.1 37400
-
 gel filtration Cereibacter sphaeroides
1.15.1.1 37400
-
 gel filtration Arthrospira platensis
1.15.1.1 37400
-
 Fe-SOD Arthrospira platensis
1.15.1.1 37400
-
 Mn-SOD Cereibacter sphaeroides

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.15.1.1 dimethyl sulfoxide stable up to 55% v/v Arthrospira platensis
1.15.1.1 dimethyl sulfoxide stable up to 70% v/v Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Arthrospira platensis
-
 Fe-SOD
-
1.15.1.1 Arthrospira platensis Fe-SOD
-
 Fe-SOD
-
1.15.1.1 Cereibacter sphaeroides
-
 aerobically grown
-
1.15.1.1 Cereibacter sphaeroides
-
 Mn-SOD
-
1.15.1.1 Cereibacter sphaeroides Mn-SOD
-
 Mn-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 Fe-SOD Arthrospira platensis
1.15.1.1 Mn-SOD Cereibacter sphaeroides

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Cereibacter sphaeroides
a
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Arthrospira platensis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
 Cereibacter sphaeroides O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Arthrospira platensis O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Arthrospira platensis Fe-SOD O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Cereibacter sphaeroides Mn-SOD O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 18300, Mn-SOD, SDS-PAGE Cereibacter sphaeroides
1.15.1.1 dimer 2 * 18100, Fe-SOD, SDS-PAGE Arthrospira platensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 70
-
 half-life: 6.5 min Arthrospira platensis
1.15.1.1 70
-
 half-life: 12.75 min Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.8
-
 Mn-SOD Cereibacter sphaeroides
1.15.1.1 7.8
-
 Fe-SOD Arthrospira platensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 3 10.8 30 min, pH 3.0: about 30% loss of activity, pH 10.8: rapid inactivation above Cereibacter sphaeroides
1.15.1.1 5 10.8 30 min, unstable below pH 5.5 and above pH 10.8 Arthrospira platensis