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Literature summary extracted from
- Superoxide dismutase from Thermus aquaticus. Isolation and characterisation of manganese and apo enzymes (1977), Eur. J. Biochem., 73, 373-381.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.15.1.1 | guanidinium chloride: 6 M, pH 7.5, 16 h, 23°C, stable | Thermus aquaticus |
| 1.15.1.1 | urea: 8 M, pH 7.5, 16 h, 23°C, stable | Thermus aquaticus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | Mn2+ | 2 atoms of manganese per molecule | Thermus aquaticus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 21000 | - |
4 * 21000, Mn-SOD, SDS-PAGE | Thermus aquaticus |
| 1.15.1.1 | 80000 | - |
Mn-SOD, gel filtration | Thermus aquaticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.15.1.1 | Thermus aquaticus | - |
Mn-SOD | - |
| 1.15.1.1 | Thermus aquaticus Mn-SOD | - |
Mn-SOD | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.15.1.1 | Mn-SOD | Thermus aquaticus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.15.1.1 | reconstitution of active enzyme after withdrawal of Mn2+ by addition of Mn2+ to apoprotein in 8 M urea at acid pH | Thermus aquaticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | additional information | - |
- |
Thermus aquaticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.15.1.1 | O2- + H+ | - |
Thermus aquaticus | O2 + H2O2 | - |
? | |
| 1.15.1.1 | O2- + H+ | - |
Thermus aquaticus Mn-SOD | O2 + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | tetramer | 4 * 21000, Mn-SOD, SDS-PAGE | Thermus aquaticus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 95 | - |
10 min, stable up to | Thermus aquaticus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 4 | 10.5 | 12 h, 23°C, stable | Thermus aquaticus |
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