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Literature summary extracted from

  • Ghosh, S.; Sadhukhan, P.C.; Chaudhuri, J.; Ghosh, D.K.; Mandal, A.
    Purification and properties of mercuric reductase from Azotobacter chroococcum (1999), J. Appl. Microbiol., 86, 7-12.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.16.1.1 2-mercaptoethanol required Azotobacter chroococcum
1.16.1.1 2-mercaptoethanol optimal concentration: 2 mM Azotobacter chroococcum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.16.1.1 Ag+ 0.1 mM Azotobacter chroococcum
1.16.1.1 Bi3+ 0.1 mM, 40% inhibition Azotobacter chroococcum
1.16.1.1 Cd2+
-
 Azotobacter chroococcum
1.16.1.1 CoCl2
-
 Azotobacter chroococcum
1.16.1.1 Cu2+ 0.1 mM, 44% inhibition Azotobacter chroococcum
1.16.1.1 HgCl2 activity is inhibited by an excess of HgCl2 Azotobacter chroococcum
1.16.1.1 NaN3
-
 Azotobacter chroococcum
1.16.1.1 NEM 2 mM, 60% inhibition Azotobacter chroococcum
1.16.1.1 Ni2+ 0.1 mM, 62% inhibition Azotobacter chroococcum
1.16.1.1 Pb2+ 0.1 mM, 60% inhibition Azotobacter chroococcum
1.16.1.1 Zn2+ 0.1 mM, 60% inhibition Azotobacter chroococcum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.1.1 0.011
-
 Hg
-
 Azotobacter chroococcum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.16.1.1 Mg2+ required Azotobacter chroococcum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.16.1.1 54000
-
 1 * 54000 + 1 * 69000, SDS-PAGE Azotobacter chroococcum
1.16.1.1 69000
-
 1 * 54000 + 1 * 69000, SDS-PAGE Azotobacter chroococcum
1.16.1.1 142000
-
 gel filtration Azotobacter chroococcum

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.1 Azotobacter chroococcum
-
 SS2
-
1.16.1.1 Azotobacter chroococcum SS2
-
 SS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.1.1
-
 Azotobacter chroococcum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16.1.1 18.32
-
-
 Azotobacter chroococcum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.1 Hg + NADP+ + H+
-
 Azotobacter chroococcum Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+
-
 Azotobacter chroococcum SS2 Hg2+ + NADPH
-
 r
1.16.1.1 Hg2+ + NADPH
-
 Azotobacter chroococcum Hg + NADP+ + H+
-
 ?
1.16.1.1 Hg2+ + NADPH
-
 Azotobacter chroococcum SS2 Hg + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.16.1.1 dimer 1 * 54000 + 1 * 69000, SDS-PAGE Azotobacter chroococcum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.16.1.1 45
-
-
 Azotobacter chroococcum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.16.1.1 7.4
-
-
 Azotobacter chroococcum

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.1 NADPH
-
 Azotobacter chroococcum