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Literature summary extracted from

  • Blaghen, M.; Vidon, D.J.M.; El Kebbaj, M.S.
    Purification and properties of mercuric reductase from Yersinia enterocolitica 138A14 (1993), Can. J. Microbiol., 39, 193-200.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.16.1.1 2-mercaptoethanol required for activity with merthiolate Yersinia enterolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.16.1.1 Ag2+
-
 Yersinia enterolytica
1.16.1.1 Cd2+ weak inhibition Yersinia enterolytica
1.16.1.1 Cu2+
-
 Yersinia enterolytica
1.16.1.1 HgCl2 activity is inhibited by an excess of HgCl2 Yersinia enterolytica
1.16.1.1 Mn2+ weak inhibition Yersinia enterolytica
1.16.1.1 Ni2+
-
 Yersinia enterolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.1.1 additional information
-
 additional information the Km-value for HgCl2 is dependent on the concentration of exogenous thiol compounds Yersinia enterolytica
1.16.1.1 0.2
-
 Hg 20 mM 2-mercaptoethanol Yersinia enterolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.16.1.1 70000
-
 3 * 70000, SDS-PAGE Yersinia enterolytica
1.16.1.1 200000
-
 gel filtration Yersinia enterolytica

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.1 Yersinia enterolytica
-
 138A14
-
1.16.1.1 Yersinia enterolytica 138A14
-
 138A14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.1.1
-
 Yersinia enterolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16.1.1 2.08
-
-
 Yersinia enterolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.1 Hg + NADP+ + H+
-
 Yersinia enterolytica Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+
-
 Yersinia enterolytica 138A14 Hg2+ + NADPH
-
 r
1.16.1.1 Hg2+ + NADPH
-
 Yersinia enterolytica Hg + NADP+ + H+
-
 ?
1.16.1.1 Hg2+ + NADPH
-
 Yersinia enterolytica 138A14 Hg + NADP+ + H+
-
 ?
1.16.1.1 merthiolate + NADPH
-
 Yersinia enterolytica ? + NADP+
-
 ?
1.16.1.1 merthiolate + NADPH
-
 Yersinia enterolytica 138A14 ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.16.1.1 More along with ageing, as well as limited proteolytic digestion, the enzyme evolves to give a dimeric molecule of 105000 Da composed of two identical subunits of 52000 Da Yersinia enterolytica
1.16.1.1 trimer 3 * 70000, SDS-PAGE Yersinia enterolytica

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.1 FAD contains FAD Yersinia enterolytica
1.16.1.1 NADPH
-
 Yersinia enterolytica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.16.1.1 0.016
-
 Ag2+
-
 Yersinia enterolytica
1.16.1.1 0.0175
-
 Ni2+
-
 Yersinia enterolytica
1.16.1.1 0.018
-
 Cu2+
-
 Yersinia enterolytica