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Literature summary extracted from

  • Booth, J.E.; Williams, J.W.
    The isolation of a mercuric ion-reducing flavoprotein from Thiobacillus ferrooxidans (1984), J. Gen. Microbiol., 130, 725-730.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.16.1.1 Ag+ AgNO3 Acidithiobacillus ferrooxidans
1.16.1.1 CoCl2 CoCl2 Acidithiobacillus ferrooxidans
1.16.1.1 Cu2+ CuCl2 Acidithiobacillus ferrooxidans
1.16.1.1 CuCl
-
 Acidithiobacillus ferrooxidans
1.16.1.1 FeCl3
-
 Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.1.1 0.0089
-
 Hg
-
 Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.16.1.1 54000
-
 x * 54000 + x * 62000, SDS-PAGE Acidithiobacillus ferrooxidans
1.16.1.1 62000
-
 x * 54000 + x * 62000, SDS-PAGE Acidithiobacillus ferrooxidans
1.16.1.1 130000
-
 gel filtration Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.1 Acidithiobacillus ferrooxidans
-
 TFI 29
-
1.16.1.1 Acidithiobacillus ferrooxidans TFI 29
-
 TFI 29
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.1.1
-
 Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16.1.1 12.8
-
-
 Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.1 Hg + NADP+ + H+
-
 Acidithiobacillus ferrooxidans Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+
-
 Acidithiobacillus ferrooxidans TFI 29 Hg2+ + NADPH
-
 r
1.16.1.1 Hg2+ + NADPH
-
 Acidithiobacillus ferrooxidans Hg + NADP+ + H+
-
 ?
1.16.1.1 Hg2+ + NADPH
-
 Acidithiobacillus ferrooxidans TFI 29 Hg + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.16.1.1 dimer x * 54000 + x * 62000, SDS-PAGE Acidithiobacillus ferrooxidans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.16.1.1 12.4
-
 HgCl2
-
 Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.16.1.1 7.5
-
-
 Acidithiobacillus ferrooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.16.1.1 6.5 9 pH 6.5: about 60% of maximal activity, pH 9.0: about 50% of maximal activity Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.1 FAD contains FAD Acidithiobacillus ferrooxidans
1.16.1.1 NADPH
-
 Acidithiobacillus ferrooxidans