Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reichard, P.; Eliasson, R.; Ingemarson, R.; Thelander, L.
    Cross-talk between the allosteric effector-binding sites in mouse ribonucleotide reductase (2000), J. Biol. Chem., 275, 33021-33026.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP required for CDP reduction Mus musculus
1.17.4.1 dATP stimulation of ADP reduction Mus musculus
1.17.4.1 dATP 6fold stimulation of GDP reduction Mus musculus
1.17.4.1 dGTP required for ADP reduction Mus musculus
1.17.4.1 dTTP required for GDP reduction Mus musculus
1.17.4.1 additional information subunit R1 has 2 effector-binding sites per polypeptide chain: one activity site for dATP and ATP, with dATP-inhibiting and ATP-stimulating catalytic activity and a second specificity site for dATP, ATP, dTTP and dGTP directing substrate specificity Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.17.4.1 D57N mutation in R1 subunit, in contrast to wild-type dATP stimulates CDP reduction, GDP reduction is inhibited by dGTP, ADP reduction is inhibited by dTTP similar to wild-type, this suggests that the mutant enzyme binds both ATP and dATP to the activity site but does not distinguish between them when it comes to catalysis Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 dATP strong inhibition of the ATP activated enzyme, complete inhibition of GDP reduction, inhibition of ADP reduction Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Mus musculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.4.1 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin allosteric regulation of the catalytic activity of subunit R1 Mus musculus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Mus musculus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O CDP, ADP and GDP are reduced very poorly in the absence of allosteric effectors ir