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Literature summary extracted from

  • Fieschi, F.; Torrents, E.; Toulokhonova, L.; Jordan, A.; Hellman, U.; Barbe, J.; Gibert, I.; Karlsson, M.; Sjoberg, B.M.
    The manganese-containing ribonucleotide reductase of Corynebacterium ammoniagenes is a class Ib enzyme (1998), J. Biol. Chem., 273, 4329-4337.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP stimulation of CDP reduction Corynebacterium ammoniagenes
1.17.4.1 dATP stimulation of CDP reduction Corynebacterium ammoniagenes
1.17.4.1 dTTP stimulation of CDP reduction Corynebacterium ammoniagenes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 expression R1E and R2F subunits in Escherichia coli Corynebacterium ammoniagenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 Hydroxyurea 2 mM, approx. 80% inactivation Corynebacterium ammoniagenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Mn2+ EPR-silent Mn bound to the polypeptide chain, approx. 0.5 mol manganese ions/mol of R2F polypeptide Corynebacterium ammoniagenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.1 37900
-
 alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence Corynebacterium ammoniagenes
1.17.4.1 81200
-
 alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence Corynebacterium ammoniagenes
1.17.4.1 160000
-
 gel filtration Corynebacterium ammoniagenes

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Corynebacterium ammoniagenes O69273 class Ib reductase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 low-salt precipitation, DEAE-cellulose, Supredex 200, Mono Q Corynebacterium ammoniagenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 0.034
-
-
 Corynebacterium ammoniagenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin NrdH-redoxin obtained from an overproducing strain, no activity with E. coli thioredoxin Corynebacterium ammoniagenes 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.17.4.1 trimer alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence Corynebacterium ammoniagenes