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Literature summary extracted from

  • Hopper, S.
    Ribonucleotide reductase of rabbit bone marrow (1978), Methods Enzymol., 51, 237-246.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.17.4.1 glycerol and ATP required for stabilization Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 8-hydroxyquinoline 5-sulfonate no inhibition in the presence of excess iron Oryctolagus cuniculus
1.17.4.1 bathophenanthroline sulfonate no effect in the presence of excess iron Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 ammonium sulfate, Sephadex G-200, dATP-Sepharose Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.4.1 bone marrow
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 0.00837
-
-
Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.17.4.1 -15°C or -70°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiotreitol, 40% glycerol, 2 mM ATP, 6 months, no loss of activity Oryctolagus cuniculus
1.17.4.1 -15°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiotreitol, 2 mM ATP, 6 months, 80% loss of activity Oryctolagus cuniculus
1.17.4.1 -70°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiothreitol, 2 mM ATP, 6 months 60% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
Oryctolagus cuniculus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
ir