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Literature summary extracted from

  • Engström, Y.; Eriksson, S.; Thelander, L.; Akerman, M.
    Ribonucleotide reductase from calf thymus. Purification and properties (1979), Biochemistry, 18, 2941-2948.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 2'-deoxy-2'-azidocytidine diphosphate thymus enzyme, reversible inhibition Bos taurus
1.17.4.1 2'-deoxy-2'-azidocytidine diphosphate inactivation Escherichia coli
1.17.4.1 bathophenanthroline sulfonate 1.5 mM, complete inactivation after 30 min, complete reactivation with FeCl3 Bos taurus
1.17.4.1 EDTA
-
 Bos taurus
1.17.4.1 EDTA
-
 Escherichia coli
1.17.4.1 Hydroxyurea 1 mM, approx. 90% inhibition; thymus enzyme, reversible inhibition Bos taurus
1.17.4.1 Hydroxyurea
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Iron
-
 Escherichia coli
1.17.4.1 Iron nonheme iron is an essential component of the enzyme Bos taurus
1.17.4.1 Mg2+
-
 Escherichia coli
1.17.4.1 Mg2+ thymus enzyme: about 50% activity in absence of added Mg2+, optimal Mg2+ concentration varies with concentration of nucleotide effector Bos taurus
1.17.4.1 additional information salt-dependence of calf thymus enzyme: optimal activity in 40 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer, pH 7.6, in the presence of 80-120 mM KCl, precipitation in lower salt concentration, inhibition in higher salt concentration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Bos taurus
-
 calf
-
1.17.4.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 streptomycin, ammonium sulfate, DEAE-cellulose, hydroxylapatite, dATP-Sepharose Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.4.1 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 additional information
-
 optimal activity in 40 mM 4-(2-hydroxyethyl)-1-piperazineethansulfonic acid, pH 7.6, 80-120 mM KCl Bos taurus
1.17.4.1 0.0237
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.17.4.1 -70°, 50 mM Tris-HCl, pH 7.6, 100 mM KCl, several months, no loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Bos taurus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.4.1 25 37 reaction rate is about twice as fast at 37°C as the rate at 25°C Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.4.1 0.023
-
 2'-deoxy-2'-azidocytidine diphosphate
-
 Bos taurus