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Literature summary extracted from

  • Vitols, E.; Bauer, V.A.; Stanbrough, E.C.
    Ribonucleotide reductase from Saccharomyces cerevisiae (1970), Biochem. Biophys. Res. Commun., 41, 71-77.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 phosphate 50 mM, slight stimulation, inhibition at 200 mM Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
1.17.4.1 partially purified enzyme is very unstable in solution, half-life at 0°C: less than 24 h Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 additional information
-
 Herpes simplex virus
1.17.4.1 additional information not inhibited by 8-hydroxyquinoline and o-phenanthroline Saccharomyces cerevisiae
1.17.4.1 YAGAVVNDL peptide may prevent association of the two subunits by competing for the subunit binding site Herpes simplex virus
1.17.4.1 YGAVVNDL
-
 Herpes simplex virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 0.003
-
 thioredoxin
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Herpes simplex virus
-
 type 1, HSV-1
-
1.17.4.1 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 0.0072
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.17.4.1 quick-freezing in liquid nitrogen and subsequent storage at -20°C, several weeks, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Herpes simplex virus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin maximal activity with E. coli thioredoxin Saccharomyces cerevisiae 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir