Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stubbe, J.
    Ribonucleotide reductases: amazing and confusing (1990), J. Biol. Chem., 265, 5329-5332.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP stimulation of UDP reduction Escherichia coli
1.17.4.1 ATP stimulation of CDP reduction Escherichia coli
1.17.4.1 dTTP stimulation of UDP reduction Escherichia coli
1.17.4.1 dTTP stimulation of CDP reduction Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.17.4.1 C225A 4-6% of wild-type activity Escherichia coli
1.17.4.1 C225S major product formed by interaction with CDP is cytosine Escherichia coli
1.17.4.1 C439A 4-6% of wild-type activity Escherichia coli
1.17.4.1 C462S in the presence of dithiothreitol the major product formed by interaction with CDP is cytosine Escherichia coli
1.17.4.1 C754A active with dithiothreitol as reductant, 3% of wild type activity with thioredoxin Escherichia coli
1.17.4.1 C759A active with dithiothreitol as reductant, 3% of wild type activity with thioredoxin Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 CDP competitive inhibition of UDP reduction Escherichia coli
1.17.4.1 dATP inhibition of CDP reduction; inhibition of UDP reduction Escherichia coli
1.17.4.1 additional information mechanism-based inhibitors Escherichia coli
1.17.4.1 UDP competitive inhibition of CDP reduction Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Iron B2 subunit contains 2 dinuclear Fe3+ centers Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.4.1 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin proposed mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.4.1 3.33
-
 CDP activity of subunit B1 assayed in the presence of an excess of subunit B2 Escherichia coli
1.17.4.1 12.9
-
 CDP activity of subunit B2 assayed in the presence of an excess of subunit B1 Escherichia coli