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Literature summary extracted from

  • Tauer, A.; Benner, S.A.
    The B12-dependent ribonucleotide reductase from the archaebacterium Thermoplasma acidophila: an evolutionary solution to the ribonucleotide reductase conundrum (1997), Proc. Natl. Acad. Sci. USA, 94, 53-58.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 5'-deoxyadenosylcobalamin
-
 Thermoplasma acidophilum
1.17.4.2 5'-deoxyadenosylcobalamin
-
 Lactobacillus leichmannii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.2 chromosomal DNA containg RNR gene PCR amplified, gene cloned, completely sequenced and expressed in Escherichia coli Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.2 100000
-
 size exclusion chromatography, SDS-PAGE Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus aquaticus
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Escherichia coli
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Homo sapiens
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Halobacterium salinarum
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Haloferax volcanii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermoplasma acidophilum
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Mycobacterium tuberculosis
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Anabaena sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus aquaticus X1
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Anabaena sp.
-
-
-
1.17.4.2 Escherichia coli
-
-
-
1.17.4.2 Halobacterium salinarum
-
-
-
1.17.4.2 Haloferax volcanii
-
-
-
1.17.4.2 Homo sapiens
-
 human
-
1.17.4.2 Lactobacillus leichmannii
-
-
-
1.17.4.2 Mycobacterium tuberculosis
-
-
-
1.17.4.2 Thermoplasma acidophilum
-
 DSM 1728
-
1.17.4.2 Thermus aquaticus
-
-
-
1.17.4.2 Thermus aquaticus X1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2
-
 Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.2 0.0018
-
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Thermus aquaticus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Homo sapiens 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Halobacterium salinarum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Haloferax volcanii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Thermoplasma acidophilum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Mycobacterium tuberculosis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Anabaena sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Thermus aquaticus X1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermus aquaticus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Homo sapiens 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Halobacterium salinarum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Haloferax volcanii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermoplasma acidophilum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Mycobacterium tuberculosis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Anabaena sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermus aquaticus X1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.2 55
-
-
 Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 coenzyme B12
-
 Thermoplasma acidophilum
1.17.4.2 coenzyme B12
-
 Mycobacterium tuberculosis
1.17.4.2 coenzyme B12
-
 Lactobacillus leichmannii