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Literature summary extracted from

  • Halicky, P.; Kollarova, M.; Kois, P.; Zelinka, J.
    Immobilization of ribonucleotide reductase from Streptomyces aureofaciens (1989), Collect. Czech. Chem. Commun., 54, 2528-2541.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 dATP specific activator for CTP reduction Kitasatospora aureofaciens
1.17.4.2 dGTP specific activator for ATP reduction Kitasatospora aureofaciens

General Stability

EC Number General Stability Organism
1.17.4.2 immobilization stabilizes the enzyme substantially, compared with free enzyme, which has an inactivation half-life of less than 1 day, on immobilization the inactivation half-life is about 15 days Kitasatospora aureofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.2 0.01
-
 CTP immobilized enzyme Kitasatospora aureofaciens
1.17.4.2 0.01
-
 UTP immobilized enzyme Kitasatospora aureofaciens
1.17.4.2 0.015
-
 ATP immobilized enzyme Kitasatospora aureofaciens
1.17.4.2 0.07
-
 ATP in presence of effector dGTP Kitasatospora aureofaciens
1.17.4.2 0.08
-
 ADP in presence of effector dGTP Kitasatospora aureofaciens
1.17.4.2 0.085
-
 CTP presence of effector dATP Kitasatospora aureofaciens
1.17.4.2 0.09
-
 GTP immobilized enzyme Kitasatospora aureofaciens
1.17.4.2 0.11
-
 CDP presence of effector dATP Kitasatospora aureofaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.2 Ca2+ markedly stimulates activity Kitasatospora aureofaciens
1.17.4.2 Mg2+ markedly stimulates activity Kitasatospora aureofaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Kitasatospora aureofaciens key enzyme in the pathway of DNA biosynthesis in all organisms 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Kitasatospora aureofaciens
-
 industrial strain BMK
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.4.2 mycelium
-
 Kitasatospora aureofaciens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 ADP + reduced thioredoxin
-
 Kitasatospora aureofaciens dADP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ATP + reduced thioredoxin
-
 Kitasatospora aureofaciens dATP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced dithiothreitol
-
 Kitasatospora aureofaciens dCDP + oxidized dithiothreitol + H2O
-
 r
1.17.4.2 CTP + reduced thioredoxin
-
 Kitasatospora aureofaciens dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 GTP + reduced thioredoxin
-
 Kitasatospora aureofaciens dGTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + dithioerythritol
-
 Kitasatospora aureofaciens 2'-deoxyribonucleoside triphosphate + ? + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Kitasatospora aureofaciens 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O key enzyme in the pathway of DNA biosynthesis in all organisms Kitasatospora aureofaciens 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 UTP + reduced thioredoxin
-
 Kitasatospora aureofaciens dUTP + oxidized thioredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.2 monomer
-
 Kitasatospora aureofaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.2 30
-
-
 Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.2 8 8.5
-
 Kitasatospora aureofaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.4.2 6.4 9.6
-
 Kitasatospora aureofaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 coenzyme B12
-
 Kitasatospora aureofaciens