Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sando, G.N.; Hogenkamp, H.P.C.
    Ribonucleotide reductase from Thermus X-1, a thermophilic organism (1973), Biochemistry, 12, 3316-3322.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 5'-deoxy-5'-adenosylcobalamin
-
Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.2 0.29
-
GTP at 37°C Thermus sp.
1.17.4.2 0.32
-
CTP at 37°C Thermus sp.
1.17.4.2 0.99
-
GTP at 70°C Thermus sp.
1.17.4.2 1
-
ATP at 70°C Thermus sp.
1.17.4.2 1.4
-
CTP at 70°C Thermus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.2 Mg2+ stimulates enzyme activity Thermus sp.
1.17.4.2 Mn2+ stimulates enzyme activity Thermus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.2 80000
-
gel filtration Thermus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus aquaticus
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus sp.
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus aquaticus YT-1
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Lactobacillus leichmannii
-
-
-
1.17.4.2 Thermus aquaticus
-
-
-
1.17.4.2 Thermus aquaticus YT-1
-
-
-
1.17.4.2 Thermus sp.
-
X-1
-
1.17.4.2 Thermus sp. X-1
-
X-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2
-
Lactobacillus leichmannii
1.17.4.2 partially Thermus sp.

Storage Stability

EC Number Storage Stability Organism
1.17.4.2 20°C, 0.03 M dimethylglutarate buffer, pH 7.2, remains fully active on storage for 24 h Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 ADP + reduced thioredoxin
-
Thermus sp. dADP + oxidized thioredoxin + H2O
-
?
1.17.4.2 ADP + reduced thioredoxin
-
Lactobacillus leichmannii dADP + oxidized thioredoxin + H2O
-
?
1.17.4.2 ATP + reduced thioredoxin
-
Thermus sp. dATP + oxidized thioredoxin + H2O
-
?
1.17.4.2 ATP + reduced thioredoxin
-
Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
?
1.17.4.2 ATP + reduced thioredoxin
-
Thermus sp. X-1 dATP + oxidized thioredoxin + H2O
-
?
1.17.4.2 CDP + reduced thioredoxin
-
Thermus sp. dCDP + oxidized thioredoxin + H2O
-
?
1.17.4.2 CDP + reduced thioredoxin
-
Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
?
1.17.4.2 CMP + reduced thioredoxin
-
Thermus sp. dCMP + oxidized thioredoxin + H2O
-
?
1.17.4.2 CMP + reduced thioredoxin
-
Lactobacillus leichmannii dCMP + oxidized thioredoxin + H2O
-
?
1.17.4.2 CTP + reduced thioredoxin
-
Thermus sp. dCTP + oxidized thioredoxin + H2O
-
?
1.17.4.2 CTP + reduced thioredoxin
-
Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
?
1.17.4.2 CTP + reduced thioredoxin
-
Thermus sp. X-1 dCTP + oxidized thioredoxin + H2O
-
?
1.17.4.2 GDP + reduced thioredoxin
-
Thermus sp. dGDP + oxidized thioredoxin + H2O
-
?
1.17.4.2 GDP + reduced thioredoxin
-
Lactobacillus leichmannii dGDP + oxidized thioredoxin + H2O
-
?
1.17.4.2 GTP + reduced thioredoxin
-
Thermus sp. dGTP + oxidized thioredoxin + H2O
-
?
1.17.4.2 GTP + reduced thioredoxin
-
Lactobacillus leichmannii dGTP + oxidized thioredoxin + H2O
-
?
1.17.4.2 GTP + reduced thioredoxin
-
Thermus sp. X-1 dGTP + oxidized thioredoxin + H2O
-
?
1.17.4.2 IDP + reduced thioredoxin
-
Thermus sp. dIDP + oxidized thioredoxin
-
?
1.17.4.2 ITP + reduced thioredoxin
-
Thermus sp. dITP + oxidized thioredoxin
-
?
1.17.4.2 additional information GMP and AMP are no substrates Thermus sp. ?
-
?
1.17.4.2 additional information GMP and AMP are no substrates Thermus sp. X-1 ?
-
?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
Thermus aquaticus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
Thermus sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
Thermus sp. X-1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
Thermus aquaticus YT-1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Thermus aquaticus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Thermus sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Thermus aquaticus YT-1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
1.17.4.2 UDP + reduced thioredoxin
-
Thermus sp. dUDP + oxidized thioredoxin + H2O
-
?
1.17.4.2 UDP + reduced thioredoxin
-
Lactobacillus leichmannii dUDP + oxidized thioredoxin + H2O
-
?
1.17.4.2 UMP + reduced thioredoxin
-
Thermus sp. dUMP + oxidized thioredoxin + H2O
-
?
1.17.4.2 UTP + reduced thioredoxin
-
Thermus sp. dUTP + oxidized thioredoxin + H2O
-
?
1.17.4.2 UTP + reduced thioredoxin
-
Lactobacillus leichmannii dUTP + oxidized thioredoxin + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.17.4.2 monomer
-
Lactobacillus leichmannii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.2 50
-
-
Lactobacillus leichmannii
1.17.4.2 70
-
-
Thermus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.4.2 20 60
-
Lactobacillus leichmannii
1.17.4.2 20 80
-
Thermus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.2 7.8 8.4
-
Thermus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 coenzyme B12 adenosylcobalamin Lactobacillus leichmannii