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Literature summary extracted from
- Isolation and properties of reduced nicotinamide adenine dinucleotiderubredoxin oxidoreductase of Clostridium acetobutylicum (1979), Biochem. Biophys. Res. Commun., 91, 1258-1265.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.1.1 | 1,10-phenanthroline | - |
Clostridium acetobutylicum |  |
| 1.18.1.1 | 2,4-dinitrophenol | - |
Clostridium acetobutylicum | |
| 1.18.1.1 | AgNO3 | - |
Clostridium acetobutylicum | |
| 1.18.1.1 | dicoumarol | - |
Clostridium acetobutylicum | |
| 1.18.1.1 | NEM | - |
Clostridium acetobutylicum | |
| 1.18.1.1 | p-hydroxymercuribenzoate | - |
Clostridium acetobutylicum | |
| 1.18.1.1 | Quinacrine | - |
Clostridium acetobutylicum | |
| 1.18.1.1 | sodium arsenite | - |
Clostridium acetobutylicum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.1 | 38000 | - |
gel filtration | Clostridium acetobutylicum |
| 1.18.1.1 | 41000 | - |
1 * 41000, SDS-PAGE | Clostridium acetobutylicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.18.1.1 | Clostridium acetobutylicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.18.1.1 | - |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.1 | 46 | - |
rubredoxin-dependent reduction of cytochrome c | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.1.1 | 2 ferricyanide + NADH | - |
Clostridium acetobutylicum | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 1.18.1.1 | 2 ferricytochrome c + NADH | weak activity | Clostridium acetobutylicum | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
| 1.18.1.1 | additional information | enzyme catalyzes rubredoxin-dependent reduction of cytochrome c in presence of NADH | Clostridium acetobutylicum | ? | - |
? | |
| 1.18.1.1 | NADH + 2,6-dichloroindophenol | - |
Clostridium acetobutylicum | NAD+ + reduced 2,6-dichloroindophenol | - |
? | |
| 1.18.1.1 | NADH + 2-methyl-1,4-naphthoquinone | - |
Clostridium acetobutylicum | NAD+ + 2-methyl-1,4-naphthoquinol | - |
? | |
| 1.18.1.1 | NADH + H+ + 1,4-naphthoquinone | - |
Clostridium acetobutylicum | NAD+ + 1,4-naphthoquinol | - |
? | |
| 1.18.1.1 | NADH + metmyoglobin | - |
Clostridium acetobutylicum | NAD+ + reduced metmyoglobin | - |
? | |
| 1.18.1.1 | NADH + nitroblue tetrazolium | weak activity | Clostridium acetobutylicum | NAD+ + reduced nitroblue tetrazolium | - |
? | |
| 1.18.1.1 | NADH + oxidized rubredoxin | - |
Clostridium acetobutylicum | NAD+ + reduced rubredoxin | - |
? | |
| 1.18.1.1 | NADH + p-benzoquinone | - |
Clostridium acetobutylicum | NAD+ + p-benzoquinol | - |
? | |
| 1.18.1.1 | NADH + p-iodonitrotetrazolium | - |
Clostridium acetobutylicum | NAD+ + reduced p-iodonitrotetrazolium | - |
? | |
| 1.18.1.1 | NADH + p-toluoquinone | - |
Clostridium acetobutylicum | NAD+ + p-toluoquinol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.18.1.1 | monomer | 1 * 41000, SDS-PAGE | Clostridium acetobutylicum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.1.1 | FAD | prosthetic group | Clostridium acetobutylicum | |
| 1.18.1.1 | NADH | - |
Clostridium acetobutylicum | |
| 1.18.1.1 | NADPH | no significant reaction with | Clostridium acetobutylicum | |
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