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Literature summary extracted from

  • Fauque, G.D.; Klimmek, O.; Kroger, A.
    Sulfur reductases from spirilloid mesophilic sulfur-reducing eubacteria (1994), Methods Enzymol., 243, 367-383.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.98.4 GSH
-
 Sulfurospirillum deleyianum
1.12.98.4 additional information no cytochrome c3, c7 or b involved Wolinella succinogenes
1.12.98.4 additional information no cytochrome c3, c7 or b involved Sulfurospirillum deleyianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.98.4 membrane
-
 Sulfurospirillum deleyianum 16020
-
1.12.98.4 membrane oriented toward the periplasmic side Wolinella succinogenes 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.4 Fe iron-sulfur (non-heme) protein Wolinella succinogenes
1.12.98.4 Fe iron-sulfur (non-heme) protein Sulfurospirillum deleyianum
1.12.98.4 Fe 0.12 mM nonheme iron per g protein, Psr B (Fe-S)-centers Wolinella succinogenes
1.12.98.4 Mo enzym contains molybdenum 0.006 mmol/g protein bound to a pterin dinucleotide Wolinella succinogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.4 sulfur Wolinella succinogenes reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms H2S
-
 ?
1.12.98.4 sulfur Sulfurospirillum deleyianum reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms H2S
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.12.98.4 Triton X-100
-
 Wolinella succinogenes
1.12.98.4 Triton X-100
-
 Sulfurospirillum deleyianum

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.4 Sulfurospirillum deleyianum
-
-
-
1.12.98.4 Wolinella succinogenes
-
 DSM 1740
-
1.12.98.4 Wolinella succinogenes
-
 enzyme formed during growth at the expense of sulfur reduction by formate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.4
-
 Sulfurospirillum deleyianum
1.12.98.4 isolation and incorporation into liposomes Wolinella succinogenes

Reaction

EC Number Reaction Comment Organism Reaction ID
1.12.98.4 H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 sulfur can be reduced with hydrogen as donor in the presence of hydrogenase Wolinella succinogenes
a
1.12.98.4 H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 sulfur can be reduced with hydrogen as donor in the presence of hydrogenase Sulfurospirillum deleyianum
a
1.12.98.4 H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 polysulfide appears to be the soluble intermediate in the sulfur reduction Wolinella succinogenes
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.4 0.27
-
 crude extract Sulfurospirillum deleyianum
1.12.98.4 732
-
-
 Wolinella succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.4 additional information sulfur can be reduced by photochemical reduction in the presence of phenosafranin Sulfurospirillum deleyianum ?
-
 ?
1.12.98.4 organic trisulfides + electron donor
-
 Sulfurospirillum deleyianum H2S + oxidized electron donor
-
 ?
1.12.98.4 polysulfide + electron donor
-
 Sulfurospirillum deleyianum H2S + oxidized electron donor
-
 ?
1.12.98.4 polysulfide + electron donor polysulfide appears to be the soluble intermediate in the sulfur reduction, electron-transport-coupled phosphorylation Wolinella succinogenes H2S + oxidized electron donor
-
 ?
1.12.98.4 sulfur reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms Wolinella succinogenes H2S
-
 ?
1.12.98.4 sulfur reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms Sulfurospirillum deleyianum H2S
-
 ?
1.12.98.4 sulfur + electron donor
-
 Sulfurospirillum deleyianum H2S + oxidized electron donor
-
 ?
1.12.98.4 sulfur + electron donor electron donor: molecular hydrogen Wolinella succinogenes H2S + oxidized electron donor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.4 More SDS-PAGE, Psr A: 85000 Da and Psr B: 23000 Da, amount to approximately 70% of the total protein and are present at nearly equimolar quantities, Psr C not identified Wolinella succinogenes

Synonyms

EC Number Synonyms Comment Organism
1.12.98.4 polysulfide reductase Psr A, Psr B, Psr C Wolinella succinogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.4 8.7 8.9
-
 Sulfurospirillum deleyianum