EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.32 | EDTA | 2 mM, 45% inhibition, complete inhibition after preincubation with 1 mM EDTA for 30 min, almost the total activity can be restored with 2 mM Co2+, Ni2+ and Mn2+ | Rauvolfia serpentina | |
1.5.1.32 | p-hydroxymercuribenzoate | 0.2 mM, complete inhibition | Rauvolfia serpentina |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.32 | additional information | most probably a metalloprotein, complete inhibition after preincubation with 1 mM EDTA for 30 min, can be restored with 2 mM Co2+, Ni2+ and Mn2+ | Rauvolfia serpentina |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.32 | 43000 | - |
gel filtration | Rauvolfia serpentina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.32 | vomilenine + NADPH | Rauvolfia serpentina | enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.32 | Rauvolfia serpentina | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.32 | ammonium sulfate, Source 30Q, Source 15Phe, 2',5'-ADP-Sepharose | Rauvolfia serpentina |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.32 | cell suspension culture | - |
Rauvolfia serpentina | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.32 | 0.0031 | - |
- |
Rauvolfia serpentina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.32 | vomilenine + NADPH | - |
Rauvolfia serpentina | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | non-natural 2alpha-(S)-1,2-dihydrovomilenine is never observed | ? | |
1.5.1.32 | vomilenine + NADPH | enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline | Rauvolfia serpentina | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.32 | monomer | 1 * 43000, SDS-PAGE | Rauvolfia serpentina |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.32 | 30 | - |
50% activity at 45°C | Rauvolfia serpentina |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.32 | 6 | - |
in phosphate buffer, 50% activity at pH 5.2 and pH 6.8 respectively | Rauvolfia serpentina |
1.5.1.32 | 6.5 | - |
in phosphate/citric acid buffer | Rauvolfia serpentina |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.32 | 5.2 | 6.8 | - |
Rauvolfia serpentina |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.32 | NADPH | - |
Rauvolfia serpentina |