Any feedback?
Literature summary extracted from
- Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins (1984), J. Biol. Chem., 259, 14121-14127.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.4.1 | 0.0085 | - |
dihydroneopterin triphosphate | - |
Drosophila melanogaster |  |
| 1.5.4.1 | 1.8 | - |
glutathione | - |
Drosophila melanogaster | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.4.1 | 24000 | - |
2 * 24000, SDS-PAGE | Drosophila melanogaster |
| 1.5.4.1 | 48000 | - |
gel filtration | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.4.1 | 6-pyruvoyltetrahydropterin + glutathione | Drosophila melanogaster | enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase | glutathione disulfide + pyrimidodiazepine | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.4.1 | Drosophila melanogaster | - |
wild-type flies | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.4.1 | - |
Drosophila melanogaster |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.4.1 | head | - |
Drosophila melanogaster | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.4.1 | 0.8 | - |
- |
Drosophila melanogaster |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.5.4.1 | -80°C, enzyme purified through the Affi-Gel blue step is stable for at least 9 months | Drosophila melanogaster |
| 1.5.4.1 | -80°C, enzyme purified through the final step stable for at least 1-2 weeks | Drosophila melanogaster |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.4.1 | 6-pyruvoyltetrahydropterin + glutathione | - |
Drosophila melanogaster | glutathione disulfide + pyrimidodiazepine | - |
r | |
| 1.5.4.1 | 6-pyruvoyltetrahydropterin + glutathione | enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase | Drosophila melanogaster | glutathione disulfide + pyrimidodiazepine | - |
r | |
| 1.5.4.1 | dihydroneopterin triphosphate + glutathione | - |
Drosophila melanogaster | ? | - |
? | |
| 1.5.4.1 | glutathione disulfide + pyrimidodiazepine | - |
Drosophila melanogaster | 6-pyruvoyltetrahydropterin + glutathione | - |
r | |
| 1.5.4.1 | pyruvoyltetrahydropterin + glutathione | - |
Drosophila melanogaster | 2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b]-[1,4]diazepine | PDA | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.4.1 | dimer | 2 * 24000, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.4.1 | 30 | - |
- |
Drosophila melanogaster |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
