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Literature summary extracted from

  • Boulton, C.A.; Haywood, G.W.; Large, P.J.
    N-Methylglutamate dehydrogenase, a flavohaemoprotein purified from a new pink trimethylamine utilizing bacterium (1980), J. Gen. Microbiol., 117, 293-304.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.5 0.2
-
 2,6-dichlorophenolindophenol
-
 bacterium AT2
1.5.99.5 0.333
-
 N-Methyl-L-glutamate
-
 bacterium AT2

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.99.5 cytosol
-
 bacterium AT2 5829
-
1.5.99.5 membrane
-
 Pseudomonas sp. 16020
-
1.5.99.5 membrane
-
 Aminobacter aminovorans 16020
-
1.5.99.5 membrane
-
 bacterium AT2 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.5 108000
-
 4 * 108000 bacterium AT2
1.5.99.5 407000
-
 gel filtration bacterium AT2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Pseudomonas sp.
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Aminobacter aminovorans
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Pseudomonas methylica
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Hyphomicrobium vulgare
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O bacterium AT2
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Hyphomicrobium vulgare ZV
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Hyphomicrobium vulgare 3
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.5 Aminobacter aminovorans
-
-
-
1.5.99.5 Aminobacter aminovorans AM1
-
-
-
1.5.99.5 bacterium AT2
-
 pink facultative methylotroph
-
1.5.99.5 Hyphomicrobium vulgare
-
-
-
1.5.99.5 Hyphomicrobium vulgare 3
-
-
-
1.5.99.5 Hyphomicrobium vulgare ZV
-
-
-
1.5.99.5 Pseudomonas methylica
-
-
-
1.5.99.5 Pseudomonas sp.
-
-
-
1.5.99.5 Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.5
-
 bacterium AT2
1.5.99.5 partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.5 0.009
-
 specific activity after growth on trimethylamine bacterium AT2
1.5.99.5 0.0161
-
-
 Hyphomicrobium vulgare
1.5.99.5 0.02
-
-
 Aminobacter aminovorans
1.5.99.5 0.027
-
 specific activity after growth on methylamine bacterium AT2
1.5.99.5 0.0437
-
 purified enzyme bacterium AT2
1.5.99.5 0.113
-
-
 bacterium AT2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.5 additional information
-
 Pseudomonas sp. ?
-
 ?
1.5.99.5 additional information
-
 Aminobacter aminovorans ?
-
 ?
1.5.99.5 additional information not active with NAD+, ferricyanide cytochrome c or horse heart cytochrome c, no activity detected with N-methyl-D-alanine bacterium AT2 ?
-
 ?
1.5.99.5 additional information
-
 Aminobacter aminovorans AM1 ?
-
 ?
1.5.99.5 additional information
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 ?
-
 ?
1.5.99.5 N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl-DL-glutamate + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 DL-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Pseudomonas methylica L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Hyphomicrobium vulgare L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Hyphomicrobium vulgare ZV L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Hyphomicrobium vulgare 3 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methylglutamate + N,N,N,N-tetramethyl-o-phenylene diamine + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced N,N,N,N-tetramethyl-o-phenylene diamine
-
 ?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
 Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.99.5 N-methylglutamate + radical cation of 2,2'-azinodi-[3-ethylbenzthiazoline 6-sulfonate] + H2O
-
 bacterium AT2 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 bacterium AT2 aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.5 tetramer
-
 Aminobacter aminovorans
1.5.99.5 tetramer 4 * 108000 bacterium AT2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.99.5 40 50 half-life of the purified enzyme 35 min at 40°C, 10.5 min at 45°C and 1 min at 50°C bacterium AT2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.5 7
-
-
 bacterium AT2

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.5 FAD
-
 bacterium AT2
1.5.99.5 NAD+
-
 Pseudomonas methylica
1.5.99.5 NAD+
-
 Hyphomicrobium vulgare