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Literature summary extracted from

  • Hersh, L.B.; Stark, M.J.; Worthen, S.; Fiero, M.K.
    N-methylglutamate dehydrogenase: kinetic studies on the solubilized enzyme (1972), Arch. Biochem. Biophys., 150, 219-226.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.99.5 alpha-ketoglutarate
-
 Pseudomonas sp.
1.5.99.5 formaldehyde product inhibition Pseudomonas sp.
1.5.99.5 glutamate product inhibition Pseudomonas sp.
1.5.99.5 p-chloromercuriphenylsulfonate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.5 0.011
-
 phenazine methosulfate
-
 Pseudomonas sp.
1.5.99.5 0.043
-
 N-Methyl-L-glutamate
-
 Pseudomonas sp.
1.5.99.5 0.054
-
 2,6-dichlorophenolindophenol
-
 Pseudomonas sp.
1.5.99.5 0.1
-
 2-(methylamino)acetic acid
-
 Pseudomonas sp.
1.5.99.5 0.2
-
 N-methyl DL-valine
-
 Pseudomonas sp.
1.5.99.5 0.2
-
 N-methyl L-isoleucine
-
 Pseudomonas sp.
1.5.99.5 1.3
-
 N-methyl L-aspartate
-
 Pseudomonas sp.
1.5.99.5 1.4
-
 potassium ferricyanide
-
 Pseudomonas sp.
1.5.99.5 2.5
-
 N-methyl L-phenylalanine
-
 Pseudomonas sp.
1.5.99.5 6
-
 N-methyl L-alanine
-
 Pseudomonas sp.
1.5.99.5 36
-
 N-methyl L-serine
-
 Pseudomonas sp.
1.5.99.5 200
-
 sarcosine
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.99.5 membrane
-
 Pseudomonas sp. 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Pseudomonas sp.
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.5 Pseudomonas sp.
-
-
-
1.5.99.5 Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.5
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.5.99.5 -15°C, several months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.5 additional information N,N-dimethylglutamate is no substrate Pseudomonas sp. ?
-
 ?
1.5.99.5 additional information N,N-dimethylglutamate is no substrate Pseudomonas sp. MA / M.A. / ATCC 23819 ?
-
 ?
1.5.99.5 N-methyl DL-valine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. DL-valine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-isoleucine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-isoleucine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-phenylalanine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-phenylalanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl L-serine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-serine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl-glycine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methylglutamate + cytochrome c + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced cytochrome c
-
 ?
1.5.99.5 N-methylglutamate + cytochrome c + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced cytochrome c
-
 ?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.99.5 N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.99.5 N-methylglutamate + potassium ferricyanide + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced potassium ferricyanide
-
 ?
1.5.99.5 N-methylglutamate + potassium ferricyanide + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced potassium ferricyanide
-
 ?
1.5.99.5 sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?