Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roje, S.; Wang, H.; McNeil, S.D.; Raymond, R.K.; Appling, D.R.; Shachar-Hill, Y.; Bohnert, H.J.; Hanson, A.D.
    Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants (1999), J. Biol. Chem., 274, 36089-36096.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.20 S-adenosylmethionine stimulates Arabidopsis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.20 two MTHFR genes, a cDNAs encoding functional MTHFR is cloned, sequenced and expressed in Saccharomyces cerevisiae Zea mays
1.5.1.20 two MTHFR genes, two cDNAs encoding functional MTHFRs are cloned, sequenced and expressed in Saccharomyces cerevisiae at high levels Arabidopsis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.20 additional information not inhibited by S-adenosylmethionine Arabidopsis sp.
1.5.1.20 additional information not inhibited by S-adenosylmethionine Escherichia coli
1.5.1.20 additional information not inhibited by S-adenosylmethionine Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.20 cytosol
-
 Arabidopsis sp. 5829
-
1.5.1.20 cytosol
-
 Zea mays 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.20 33000
-
 4 * 33000 Escherichia coli
1.5.1.20 66000
-
 2 * 66000, SDS-PAGE Arabidopsis sp.
1.5.1.20 66400
-
 x * 66400, calculated from the nucleotide sequence Zea mays
1.5.1.20 141000
-
 gel filtration Arabidopsis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + NADH Escherichia coli
-
 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADH Arabidopsis sp. folate-mediated one-carbon metabolism 5-methyltetrahydrofolate + NAD+
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + NADH Zea mays folate-mediated one-carbon metabolism 5-methyltetrahydrofolate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.20 Arabidopsis sp.
-
 ecotype Columbia, expression in Saccharomyces cerevisiae
-
1.5.1.20 Escherichia coli
-
-
-
1.5.1.20 Zea mays Q9SE94 expression in Saccharomyces cerevisiae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.20 643fold purification of recombinant enzyme, expressed in Saccharomyces cerevisiae Arabidopsis sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.20 leaf
-
 Arabidopsis sp.
-
1.5.1.20 leaf
-
 Zea mays
-
1.5.1.20 root
-
 Arabidopsis sp.
-
1.5.1.20 root
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.20 6.92
-
 recombinant enzyme Arabidopsis sp.

Storage Stability

EC Number Storage Stability Organism
1.5.1.20 on ice, 3 h, loss of about 50% of activity Arabidopsis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + NADH
-
 Escherichia coli 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADH folate-mediated one-carbon metabolism Arabidopsis sp. 5-methyltetrahydrofolate + NAD+
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + NADH folate-mediated one-carbon metabolism Zea mays 5-methyltetrahydrofolate + NAD+
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Escherichia coli 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Arabidopsis sp. 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Zea mays 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor prefers NADH as reductant Escherichia coli 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: strong preference of NADH over NADPH Arabidopsis sp. 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: strong preference of NADH over NADPH Zea mays 5-methyltetrahydrofolate + oxidized acceptor
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.20 ? x * 66400, calculated from the nucleotide sequence Zea mays
1.5.1.20 dimer 2 * 66000, SDS-PAGE Arabidopsis sp.
1.5.1.20 homotetramer 4 * 33000 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.20 37
-
 assay at Escherichia coli
1.5.1.20 37
-
 assay at Arabidopsis sp.
1.5.1.20 37
-
 assay at Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.20 NADH
-
 Escherichia coli
1.5.1.20 NADH strong preference of NADH over NADPH as reductant Arabidopsis sp.
1.5.1.20 NADH strong preference of NADH over NADPH as reductant Zea mays