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Literature summary extracted from

  • Cheng, F.W.; Shane, B.; Stokstad, E.L.R.
    Pentaglutamate derivatives of folate as substrates for rat liver tetrahydropteroylglutamate methyltransferase and 5,10-methylenetetrahydrofolate reductase (1975), Can. J. Biochem., 53, 1020-1027.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.20 (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate inhibits at high concentrations Rattus norvegicus
1.5.1.20 S-adenosylmethionine in vivo feedback-inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.20 0.003
-
 (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Rattus norvegicus
1.5.1.20 0.023
-
 5-methyltetrahydrofolate
-
 Rattus norvegicus
2.1.1.13 0.004
-
 5-Methyl-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Rattus norvegicus
2.1.1.13 0.013
-
 5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.20 Rattus norvegicus
-
 adult male Sprague-Dawley rats, fed a Purina lab chow diet
-
2.1.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.20 more than 100fold partial purification Rattus norvegicus
2.1.1.13
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.20 liver
-
 Rattus norvegicus
-
2.1.1.13 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.20 additional information
-
 specific activity is greater with the monoglutamate substrate than with the pentaglutamate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.20 5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate + reduced acceptor equilibrium lies far in favor of 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate formation, pentaglutamate form binds to the same enzyme site as monoglutamate form Rattus norvegicus (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate + oxidized acceptor menadione as acceptor for 5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate formation ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: reduced acceptor is FADH2 Rattus norvegicus 5-methyltetrahydrofolate + oxidized acceptor product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor 5,10-methylene-5,6,7,8-tetrahydropteroylmonoglutamate as substrate Rattus norvegicus 5-methyltetrahydrofolate + oxidized acceptor product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor equilibrium lies far in favor of 5-methyltetrahydrofolate formation Rattus norvegicus 5-methyltetrahydrofolate + oxidized acceptor product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Rattus norvegicus 5-methyltetrahydrofolate + oxidized acceptor product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: NADH as reduced acceptor Rattus norvegicus 5-methyltetrahydrofolate + oxidized acceptor product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate ?
1.5.1.20 5-methyltetrahydrofolate + NAD+
-
 Rattus norvegicus 5,10-methylenetetrahydrofolate + NADH + H+
-
 r
1.5.1.20 additional information reverse reaction: not NADP+ as acceptor Rattus norvegicus ?
-
 ?
2.1.1.13 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate + L-homocysteine
-
 Rattus norvegicus ?
-
 ?
2.1.1.13 N5-methyltetrahydrofolate + L-homocysteine
-
 Rattus norvegicus tetrahydrofolate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Rattus norvegicus tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylpentaglutamate + L-homocysteine
-
 Rattus norvegicus tetrahydropteroylpentaglutamate + L-methionine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.20 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.20 6.7
-
 5-methyltetrahydrofolate and 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate as substrates Rattus norvegicus
2.1.1.13 6.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.20 6 8 pH 6: about 70% of activity maximum, pH 8: about 35% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate, about 65% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.20 FAD flavoprotein, oxidized acceptor in reverse reaction Rattus norvegicus
1.5.1.20 FADH2 flavoprotein, reduced acceptor in forward reaction Rattus norvegicus
1.5.1.20 NADH
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.20 0.04 0.051 (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Rattus norvegicus