EC Number | General Stability | Organism |
---|---|---|
1.5.1.20 | FAD stabilizes | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | additional information | vitamin B12 and methionine can repress enzyme biosynthesis | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | Escherichia coli | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | 5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | Escherichia coli 113-3 | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | 5-methyltetrahydrofolate + FAD | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.20 | Escherichia coli | - |
- |
- |
1.5.1.20 | Escherichia coli 113-3 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.20 | about 100fold purification | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | additional information | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | Escherichia coli | 5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | Escherichia coli 113-3 | 5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | under anaerobic conditions and in absence of electron acceptors, the equilibrium lies far to the 5-methyltetrahydrofolate formation, inclusion of menadione or oxygen promotes the oxidation of 5-methyltetrahydrofolate | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | under anaerobic conditions and in absence of electron acceptors, the equilibrium lies far to the 5-methyltetrahydrofolate formation, inclusion of menadione or oxygen promotes the oxidation of 5-methyltetrahydrofolate | Escherichia coli 113-3 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Escherichia coli 113-3 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | additional information | no direct activity with pyridine nucleotides | Escherichia coli | ? | - |
? | |
1.5.1.20 | additional information | no direct activity with pyridine nucleotides | Escherichia coli 113-3 | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 37 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 6.3 | 6.4 | formation of 5-methyltetrahydrofolate | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | FAD | enzyme-bound FAD | Escherichia coli | |
1.5.1.20 | FAD | flavoprotein, oxidized acceptor in reverse reaction | Escherichia coli | |
1.5.1.20 | FADH2 | flavoprotein, reduced acceptor in forward reaction | Escherichia coli | |
1.5.1.20 | additional information | no direct activity with pyridine nucleotides: NADH, NADPH | Escherichia coli |