EC Number | General Stability | Organism |
---|---|---|
2.1.1.13 | acetone inactivates | Sus scrofa |
2.1.1.13 | freezing and thawing, 40% loss of activity | Sus scrofa |
2.1.1.13 | mercaptoethanol, 0.2 M, inactivation after 2 h at 24°C or during freezing and thawing, no effect at 0°C | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | Escherichia coli | - |
5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | Sus scrofa | first step of conversion of 5,10-methylenetetrahydrofolate to methionine | 5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | Escherichia coli 113-3 | - |
5-methyltetrahydrofolate + FAD | - |
? | |
2.1.1.13 | 5-methyltetrahydrofolate + L-homocysteine | Sus scrofa | methionine biosynthesis | tetrahydrofolate + L-methionine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.20 | Escherichia coli | - |
- |
- |
1.5.1.20 | Escherichia coli 113-3 | - |
- |
- |
1.5.1.20 | Sus scrofa | - |
- |
- |
2.1.1.13 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.20 | - |
Escherichia coli |
1.5.1.20 | 20fold purification | Sus scrofa |
2.1.1.13 | - |
Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.20 | liver | - |
Sus scrofa | - |
2.1.1.13 | liver | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.13 | additional information | - |
assay method | Sus scrofa |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.20 | -15°C, several months, stable | Sus scrofa |
2.1.1.13 | 0°C, glutathione, N2-atmosphere, stable for at least a week | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | - |
Escherichia coli | 5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | first step of conversion of 5,10-methylenetetrahydrofolate to methionine | Sus scrofa | 5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + FADH2 | - |
Escherichia coli 113-3 | 5-methyltetrahydrofolate + FAD | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Sus scrofa | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Sus scrofa | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Escherichia coli 113-3 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Escherichia coli 113-3 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
2.1.1.13 | 5-methyltetrahydrofolate + L-homocysteine | methionine biosynthesis | Sus scrofa | tetrahydrofolate + L-methionine | - |
? | |
2.1.1.13 | N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Sus scrofa | tetrahydropteroylmonoglutamate + L-methionine | - |
? | |
2.1.1.13 | N5-methyltetrahydropteroyltriglutamate + L-homocysteine | - |
Sus scrofa | tetrahydropteroyltriglutamate + L-methionine | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.13 | 37 | - |
2 h, complete loss of activity | Sus scrofa |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.13 | 5 | - |
unstable | Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.13 | S-adenosyl-L-methionine | required | Sus scrofa | |
2.1.1.13 | vitamin B12 | enzyme contains a derivative of vitamin B12 as prosthetic group | Sus scrofa |