BRENDA - Enzyme Database show

A protein disulfide reductase from pea seeds

Hatch, M.D.; Turner, J.F.; Biochem. J. 76, 556-562 (1960)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.8
AgNO3
0.009 mM, 66% inhibition
Pisum sativum
1.8.1.8
arsenite
0.15 mM, complete inhibition
Pisum sativum
1.8.1.8
CdSO4
0.005 mM, 91% inhibition
Pisum sativum
1.8.1.8
CuSO4
0.015 mM, complete inhibition
Pisum sativum
1.8.1.8
HgCl2
0.002 mM, 92% inhibition
Pisum sativum
1.8.1.8
iodoacetate
0.054 mM, 57% inhibition
Pisum sativum
1.8.1.8
p-chloromercuribenzoate
0.054 mM, 88% inhibition
Pisum sativum
1.8.1.8
ZnSO4
0.003 mM, 82% inhibition
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.8
0.002
-
NADPH
-
Pisum sativum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.8
Avena sativa
-
-
-
1.8.1.8
Hordeum vulgare
-
-
-
1.8.1.8
Pisum sativum
-
-
-
1.8.1.8
Triticum aestivum
-
-
-
1.8.1.8
Vicia faba
-
-
-
1.8.1.8
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.8
-
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.8
root
-
Hordeum vulgare
-
1.8.1.8
root
-
Pisum sativum
-
1.8.1.8
root
-
Zea mays
-
1.8.1.8
seed
-
Avena sativa
-
1.8.1.8
seed
-
Hordeum vulgare
-
1.8.1.8
seed
-
Pisum sativum
-
1.8.1.8
seed
-
Triticum aestivum
-
1.8.1.8
seed
-
Vicia faba
-
1.8.1.8
seed
-
Zea mays
-
1.8.1.8
shoot
-
Hordeum vulgare
-
1.8.1.8
shoot
-
Pisum sativum
-
1.8.1.8
shoot
-
Triticum aestivum
-
1.8.1.8
shoot
-
Vicia faba
-
1.8.1.8
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.8.1.8
additional information
-
-
Pisum sativum
Storage Stability
EC Number
Storage Stability
Organism
1.8.1.8
-15C, 8 weeks, stable
Vicia faba
1.8.1.8
-15C, 8 weeks, stable
Triticum aestivum
1.8.1.8
-15C, 8 weeks, stable
Hordeum vulgare
1.8.1.8
-15C, 8 weeks, stable
Pisum sativum
1.8.1.8
-15C, 8 weeks, stable
Zea mays
1.8.1.8
-15C, 8 weeks, stable
Avena sativa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Vicia faba
NAD(P)+ + protein dithiol
-
437710
Vicia faba
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Triticum aestivum
NAD(P)+ + protein dithiol
-
437710
Triticum aestivum
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Hordeum vulgare
NAD(P)+ + protein dithiol
-
437710
Hordeum vulgare
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Zea mays
NAD(P)+ + protein dithiol
-
437710
Zea mays
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Avena sativa
NAD(P)+ + protein dithiol
-
437710
Avena sativa
?
1.8.1.8
NAD(P)H + protein disulfide
reaction rate with NADPH is approximately twice that with NADH
437710
Pisum sativum
NAD(P)+ + protein dithiol
-
437710
Pisum sativum
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.8.1.8
60
-
10 min, stable
Pisum sativum
1.8.1.8
70
-
10 min, complete inactivation
Pisum sativum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.8
6.9
7.4
-
Pisum sativum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.8.1.8
6.2
7.8
pH 6.2: about 50% of activity maximum, pH 7.8: about 20% of activity maximum
Pisum sativum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.8.1.8
5.5
-
5C or 22C, irreversible inactivation
Pisum sativum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.8
NADH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
1.8.1.8
NADPH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.8
NADH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
1.8.1.8
NADPH
reaction rate with NADPH is approximately twice of that with NADH
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.8
AgNO3
0.009 mM, 66% inhibition
Pisum sativum
1.8.1.8
arsenite
0.15 mM, complete inhibition
Pisum sativum
1.8.1.8
CdSO4
0.005 mM, 91% inhibition
Pisum sativum
1.8.1.8
CuSO4
0.015 mM, complete inhibition
Pisum sativum
1.8.1.8
HgCl2
0.002 mM, 92% inhibition
Pisum sativum
1.8.1.8
iodoacetate
0.054 mM, 57% inhibition
Pisum sativum
1.8.1.8
p-chloromercuribenzoate
0.054 mM, 88% inhibition
Pisum sativum
1.8.1.8
ZnSO4
0.003 mM, 82% inhibition
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.8
0.002
-
NADPH
-
Pisum sativum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.8
-
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.8
root
-
Hordeum vulgare
-
1.8.1.8
root
-
Pisum sativum
-
1.8.1.8
root
-
Zea mays
-
1.8.1.8
seed
-
Avena sativa
-
1.8.1.8
seed
-
Hordeum vulgare
-
1.8.1.8
seed
-
Pisum sativum
-
1.8.1.8
seed
-
Triticum aestivum
-
1.8.1.8
seed
-
Vicia faba
-
1.8.1.8
seed
-
Zea mays
-
1.8.1.8
shoot
-
Hordeum vulgare
-
1.8.1.8
shoot
-
Pisum sativum
-
1.8.1.8
shoot
-
Triticum aestivum
-
1.8.1.8
shoot
-
Vicia faba
-
1.8.1.8
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.8.1.8
additional information
-
-
Pisum sativum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.1.8
-15C, 8 weeks, stable
Vicia faba
1.8.1.8
-15C, 8 weeks, stable
Triticum aestivum
1.8.1.8
-15C, 8 weeks, stable
Hordeum vulgare
1.8.1.8
-15C, 8 weeks, stable
Pisum sativum
1.8.1.8
-15C, 8 weeks, stable
Zea mays
1.8.1.8
-15C, 8 weeks, stable
Avena sativa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Vicia faba
NAD(P)+ + protein dithiol
-
437710
Vicia faba
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Triticum aestivum
NAD(P)+ + protein dithiol
-
437710
Triticum aestivum
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Hordeum vulgare
NAD(P)+ + protein dithiol
-
437710
Hordeum vulgare
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Zea mays
NAD(P)+ + protein dithiol
-
437710
Zea mays
?
1.8.1.8
NAD(P)H + protein disulfide
-
437710
Avena sativa
NAD(P)+ + protein dithiol
-
437710
Avena sativa
?
1.8.1.8
NAD(P)H + protein disulfide
reaction rate with NADPH is approximately twice that with NADH
437710
Pisum sativum
NAD(P)+ + protein dithiol
-
437710
Pisum sativum
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.8.1.8
60
-
10 min, stable
Pisum sativum
1.8.1.8
70
-
10 min, complete inactivation
Pisum sativum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.8
6.9
7.4
-
Pisum sativum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.8.1.8
6.2
7.8
pH 6.2: about 50% of activity maximum, pH 7.8: about 20% of activity maximum
Pisum sativum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.8.1.8
5.5
-
5C or 22C, irreversible inactivation
Pisum sativum